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Khirurgii

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Year 2015 Vol. 23 No 6

GENERAL & SPECIAL SURGERY

M.B. EKTOVA1, E.P. BURLEVA 1, S.J. MEDVEDEVA 2, V.A. BAGIN 3

A COMPARATIVE ASSESSMENT OF THE SKIN AND WALL RESTRUCTURING OF THE SAPHENOUS VEIN IN CHRONIC VENOUS DISEASE CLASSES C1 AND C2

SBEE HPE "Ural State Medical University"1,
FSBES "Institute of Immunology and Physiology of Ural Branch of RAS",2
MAE "Municipal Hospital № 40"3, Yekaterinburg,
The Russian Federation

Objectives. To perform a comparative study of the skin and wall restructuring of great saphenous vein (GSV) in chronic venous diseases classes of C1 and C2 (CVD).
Methods. Tissue samples were harvested from the leg muscles of women died due to injuries. Group 1 consisted of normal samples (n=10). Groups 2 and 3 had visual signs of CVD classes C1 (n=10) and C2 (n=10). After tissue staining and description the epidermal thickness, proportion of elastic fibers (EF) and collagen fibers (CF) in the reticular dermis and the GSV media were determined. Agranulocytes, granulocytes, and mast cells (MC) were counted and optical density of MC was determined.
Results. Structural changes in the skin and GSV wall in groups C1 and C2 were of the same type with some differences. In group C1 a focal structural disorganization of EF and CF in the GSV wall with predominant changes of the endothelium without perivasculitis manifestations was observed. In group C2 the changes in the EF and CF were diffuse, involving endothelium and subendothelial structures and the media.
Perivasculitis phenomena were revealed in the skin and adventitia vessels. The number of cells involved in the chronic inflammatory process (agranulocytes, granulocytes, and mast cells) was greater in classes C1 and C2 compared to normal. A significant increase of the optical density of MC indicates to their potential to participate in modulating granulocytes and agranulocytes reactivity.
Conclusion. The chronic venous diseases classes C1 and C2 are the similar variants of genetically determined dysplasia of the connective tissue. The received data target at the implementation of a comprehensive approach in the treatment of chronic venous diseases class C1 patients.

Keywords: chronic venous diseases, C1 and C2 classes, pathomorphology of the skin and great saphenous vein wall, granulocytes, agranulocytes, reactivity, dysplasia of the connective tissue, mast cells
p. 644-650 of the original issue
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Address for correspondence:
620102, Russian Federation,
Ekaterinburg, ul. Volgogradskaya, d. 189,
MAU "Gorodskaya klinicheskaya bolnitsa № 40",
tel: 7 912 600-300-8,
7 (343) 240-14-16,
e-mail: burleva@gkb40.ur.ru,
Burleva Elena Pavlovna
Information about the authors:
Ektova M.V. A post-graduate student of the general surgery chair of SBEE HPE "Ural State Medical University", director of the medical center "AngioLine".
Burleva E.P. MD, professor of the general surgery chair of SBEE HPE "Ural State Medical University".
Medvedeva S.J. PhD, a head of the laboratory of morphology and biochemistry of FSBES "Institute of immunology and physiology of Ural branch of RAS".
Bagin V.A. PhD, a head of the Intensive Care Unit № 5 of MAE "Municipal Hospital № 40".
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