Novosti
Khirurgii

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Year 2022 Vol. 30 No 6

SCIENTIFIC PUBLICATIONS

T.À. JARKENOV 1, S.Ê. SAKHANOVA 1, M.N. ZHUMABAEV 1, S.T. ZAIROVA 2, M.M. MUKUSHEV 1, K.Z. NURMANOV 1

INFLUENCE OF POLYMORPHISMS OF GENES OF TUMOR NECROSIS FACTOR ALPHA, INTERLEUKIN-1β, INTERLEUKIN-6 AND INTERLEUKIN-10 ON THE DEVELOPMENT OF ACUTE PANCREATITIS IN THE KAZAKH POPULATION

West Kazakhstan Marat Ospanov Medical University 1,
Military Institute of Air Defense named T.J. Begeldinov 2, Aktobe,
Republic of Kazakhstan

Objective. To study the relationship of polymorphisms (rs1800629) of the tumor necrosis factor alpha gene, (rs16944) of the interleukin-1 gene, (rs1800795) of the interleukin-6 gene, (rs1800896) of the interleukin-10 gene with the development of acute pancreatitis in the Kazakh population.
Methods. The material for the study was DNA samples isolated from the peripheral blood of 96 unrelated patients with acute pancreatitis (44 men and 52 women) of Kazakh nationality. The assessment of the severity of the disease was based on the analysis of clinical and laboratory criteria. The control group included 108 (64 men and 44 women) unrelated Kazakh volunteers. Genotyping of polymorphisms (rs1800629) of the TNF-α -308 G/A gene, (rs16944) of the IL-1β -511 C/T gene, (rs1800795) of the IL-6 -174 G/C gene, (rs1800896) of the IL-10 -1082 gene A/G was carried out by real-time PCR.
Results. The genotypes of the studied polymorphisms in the control group were in accordance with the Hardy–Weinberg distribution (p>0,05). An analysis of the association of the studied genotypes with the risk of developing the disease found that the homozygous genotype -308A/A TNF-α -308 was associated with an increased risk of developing acute pancreatitis (OR=10,557, 95% CI 0,561-198,681; p=0,033). There were no significant differences in the distribution of genotypes (rs16944) of the IL-1β gene, (rs1800795) of the IL-6 gene, (rs1800896) of the IL-10 gene between patients with acute pancreatitis and control subjects (p>0,05).
Conclusion. Subjects carrying the -308A/A TNF-α, genotype have an increased risk of developing acute pancreatitis in the Kazakh population.

Keywords: àcute pancreatitis, tumour necrosis factor, Interleukin-1b, Interleukin-6, Interleukin-10, genetic polymorphism
p. 510-518 of the original issue
References
  1. Chen Y, Xie CL, Hu R, Shen CY, Zeng M, Wu CQ, Chen TW, Chen C, Tang MY, Xue HD, Jin ZY & Zhang XM. Genetic Polymorphisms: A Novel Perspective on Acute Pancreatitis. Gastroenterol Res Pract. 2017;2017:5135172. doi: 10.1155/2017/5135172
  2. Rodriguez-Nicolas A, Jiménez P, Carmona FD, et al. Association between Genetic Polymorphisms of Inflammatory Response Genes and Acute Pancreatitis. Immunol Invest. 2019;48(6):585-96. doi: 10.1080/08820139.2019.1576729
  3. Park JW, Choi JS, Han KJ, Lee SH, Kim EJ, Cho JH. Association of a genetic polymorphism of IL1RN with risk of acute pancreatitis in a Korean ethnic group. Korean J Intern Med. 2018 Nov;33(6):1103-10. doi: 10.3904/kjim.2017.133
  4. Jarkenov TA, Skipenko OG, Zhumabaev MN,. Zairova ST, Akataev NA, Yessenbaev DB, Mukushev MM Nurmanov, KZh, Balshambaev ME. The Association of Tumor Necrosis Factor-α Gene Polymorphism with the Severity of Acute Pancreatitis: a Systematic Review. Novosti Khirurgii. 2020 May-Jun; 28(3):309-17. doi: 10.22416/1382-4376-2017-27-3-27-33 (In Russ.)
  5. Liu Y, Dan G, Wu L, Chen G, Wu A, Zeng P, Xu W. Functional effect of polymorphisms in the promoter of TNFAIP3 (A20) in acute pancreatitis in the Han Chinese population. PLoS One. 2014 Jul 22;9(7):e103104. doi: 10.1371/journal.pone.0103104. eCollection 2014.
  6. Bishehsari F, Sharma A, Stello K, Toth C, O’Connell MR, Evans AC, LaRusch J, Muddana V, Papachristou GI, Whitcomb DC. TNF-alpha gene (TNFA) variants increase risk for multi-organ dysfunction syndrome (MODS) in acute pancreatitis. Pancreatology. 2012 Mar- Apr;12(2):113-8. doi: 10.1016/j.pan.2012.02.014
  7. Samgina TA, Bushueva OIu, Ivanov VP, Solodilova MA, Nazarenko PM, Polonikov AV. Sviaz’ promotornogo polimorfizma -308G>A gena faktora nekroza opukholi s tiazhest’iu techeniia ostrogo pankreatita u russkoi populiatsii zhitelei Kurskoi oblasti. Eksperim i Klin Gastroenterologiia. 2014;(9):17-20. https://elibrary.ru/item.asp?id=22677286 (In Russ.)
  8. Özhan G, Yanar HT, Ertekin C, Alpertunga B. Polymorphisms in tumour necrosis factor alpha (TNFalpha) gene in patients with acute pancreatitis. Mediators Inflamm. 2010;2010:482950. doi: 10.1155/2010/482950
  9. Park JW, Choi JS, Han KJ, Lee SH, Kim EJ, Cho JH. Association of a genetic polymorphism of IL1RN with risk of acute pancreatitis in a Korean ethnic group. Korean J Intern Med. 2018 Nov;33(6):1103-10. doi: 10.3904/kjim.2017.133
  10. Chi DZ, Chen J, Huang DP. Influence of interleukin-1β and interleukin-6 gene polymorphisms on the development of acute pancreatitis. Genet Mol Res. 2015 Feb 3;14(1):975-80. doi: 10.4238/2015.February.3.5.
  11. Samgina T.A., Zhivotova G.A., Nazarenko P.M., Polonikov A.V. The role of cytokine genetic polymorphism in development of acute pancreatitis: analysis of intergenic and environmental interactions. Russian Journal of Gastroenterology. doi: 10.22416/1382-4376-2017-27-3-27-33
  12. Jiang BZ, Tang L, Xue H, Liu DP. Role of IL-10 gene polymorphisms in the development of acute pancreatitis. Genet Mol Res. 2016;15(2):10.4238/gmr.15027743. doi: 10.4238/gmr.15027743
  13. Jiang S, Ni M, Zhang Y, Wu Y, Lu X. Association of IL-10 polymorphisms with acute pancreatitis. Int J Clin Exp Med. 2016;9(12):23702-706. https://e-century.us/files/ijcem/9/12/ijcem0038845.pdf
  14. Li D, Li J, Wang L, Zhang Q. Association between IL-1β, IL-8, and IL-10 polymorphisms and risk of acute pancreatitis. Genet Mol Res. 2015;14(2):6635-6641. doi: 10.4238/2015.June.18.6
  15. Bao XB, Ma Z, Gu JB, Wang XQ, Li HG, Wang WY. IL-8 -251T/A polymorphism is associated with susceptibility to acute pancreatitis. Genet Mol Res. 2015;14(1):1508-1514. doi: 10.4238/2015.February.20.6
Address for correspondence:
030019, Republic of Kazakhstan,
Aktobe, Maresev st. 68,
West Kazakhstan Medical
University named after Marat Ospanov,
Department of Surgical Diseases No1,
grid.443411.7,
tel.: +7 7132 544297,
e-mail: timurjarkenov@zkmu.kz,
Dzharkenov Timur A.
Information about the authors:
Jarkenov Timur A., MD, Professor of the Department of Surgical Diseases No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0003-4579-6412
Sakhanova Svetlana K., MD, Associate Professor, Head of the Scientific and Practical Center, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0001-9786-6326
Zhumabaev Marat N, Ph.D., Associate Professor, Head of the Department of Surgical Diseases No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan. https://orcid.org/0000-0002-5092-6034
Zairova Saule T, PhD, Head of the Psychological Service security, Military Institute of Air Defense Named T.J. Begeldinov, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0003-3734-6884
Mukushev Manas M , PhD, Associate Professor of the Department of Surgical Diseases No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0003-1811-3276
Nurmanov Kairat Z, Assistant of the Department of Surgical Diseases No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0003-1422-7524
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