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Khirurgii

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Year 2014 Vol. 22 No 6

OTORHINOLARYNGOLOGY

A.L. MALETS 1, S.N. RJABCEVA2, J.G. ROMANOVA1, V.P. SHORATS1

CYCLOOXYGENASE-2 ROLE IN PATHOGENESIS OF THE LARYNGEAL PAPILLOMATOSIS IN ADULTS

EE "Belarusian Medical Academy of Post-Graduate Education"1,
EE “Belarusian State Medical University” 2,
The Republic of Belarus

Objectives. On the basis of the immunohistochemical analysis to study the expression of cyclooxygenase-2 (COX-2) marker depending on the degree of dysplasia in the laryngeal papilloma tissue in adults.
Methods. The papilloma tissue fragments (62) obtained during the surgical treatment of the larynx with the tumor removal in adult patients with respiratory papillomatosis have been served as a research material. The evaluation of the dysplasia degree in the papilloma tissue was performed. The immunohistochemical assessment of expression of COX-2 monoclonal antibody in cells of the laryngeal papillomas has been conducted.
Results. The first studied group consisted of patients with laryngeal papilloma without epithelial dysplasia (7/11,3%), the second – with dysplasia of the first (mild) degree (12/19,4%), the third – with a second-degree dysplasia (moderate) (28/45,2%), the fourth – with severe dysplasia (9/14,5%), the fifth – with laryngeal cancer against a background of papillomatosis (6/9,7%). The reliable difference of the studied groups according to the weak expression intensity in the epithelial cells of laryngeal papillomas with the inverse correlation interrelation has been established. The moderate growth of expression COX-2 in the cells with dysplasia was revealed in comparison with papillomas without dysplasia. The highest figure of intensity of moderate expression and substantially equal value found in the groups of the first and second degree of epithelial dysplasia papillomas have been recorded. Reliable difference between the studied groups in severe expression intensity in cells of the laryngeal papillomas with the direct correlations has been established. The laryngeal cancer cells had the highest intensity value of severe COX-2 expression.
Conclusion. In the cells of laryngeal papillomas in adults the increase of the intensity of immunohistochemical COX-2 markers with increasing degree of dysplasia testifing the activity of this enzyme has been observed. COX-2 is considered to be an important factor in the pathogenesis of laryngeal papillomatosis in adults, and it plays one of the key roles in malignant transformation and progression of neoplasms.

Keywords: papilloma, larynx, cyclooxygenase-2, dysplasia
p. 727-734 of the original issue
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Address for correspondence:
220013, Respublika Belarus,
g. Minsk, ul. P. Brovki, d. 3, korp. 3,
GUO «Belorusskaya meditsinskaya akademiya
poslediplomnogo obrazovaniya»,
kafedra otorinolaringologii,
tel. mob.: 375 29 1020548,
e-mail: malets-al@yandex.ru,
Malets Elena Leonidovna
Information about the authors:
Malets E.L. A post-graduate student of the otorhinolaryngology chair of EE “Belarusian Medical Academy of Post-graduate Education”.
Rjabceva S.N. PhD, an assistant of the pathologic anatomy chair of EE “Belarusian State Medical University”.
Romanova J.G. PhD, associate professor of the otorhinolaryngology chair of EE “Belarusian Medical Academy of Post-graduate Education”.
Shorats O.P. An junior researcher of the research laboratory of EE “Belarusian Medical Academy of Post-graduate Education”.
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