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Khirurgii

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Year 2014 Vol. 22 No 5

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

I.V. MAIBORODIN1, V.A. MATVEEVA1, R.V.MASLOV 2, N.V. ONOPRIENKO3, I.V. KUZNETSOVA1, G.A. CHASTIKIN1

FLUORESCENT MACROPHAGES IN THE LYMPH NODES AFTER APPLICATION OF MULTIPOTENT MESENCHYMAL STROMAL CELLS WITH TRANSFECTED GFP GENE

SBEE HPE “Institute of chemical biology and fundamental medicine” SB RAS 1, Novosibirsk,
SBEE HPE “Moscow State Medical-Stomatological University named after A.I. Evdokimov” 2,
SBEE HPE “Novosibirsk State Medical University” 3,
The Russian Federation

Objectives. To determine the reactions of the regional lymph nodes, caused by implantation of the autologous multipotent mesenchymal stromal cells of the bone marrow origin (AMMSCMO) for acceleration of mandible bone defect regeneration in experiment.
Methods. The reactions of rat regional lymph nodes, caused by implantation of AMMSCBM for acceleration of bone defect regeneration of the mandible bone have been studied by the methods of fluorescent light microscopy in experiment.
Results. One week after the introduction in an injury site of the mandible bone of polyhydroxyalkanoate with adsorbed AMMSCBM with a transfected GFP gene the numerous large macrophages with a set of oval fluorescent inclusions up to 20 micrometers in diameter have been detected in the regional (submandibular) lymph nodes. In the fluorescent cells not the whole cytoplasm was glowing, but oval granules, different of sizes which reached 10 micrometers. Clusters of fluorescent cells differed in density and background color from the surrounding tissue and had a clear boundary. It gives the possibility to assume the concentration of cells with the fluorescent cytoplasm in the lymphoid nodules. On the basis of the large-sized cells of irregular shape and with the presence of inclusions and location of the lymph nodes, it was concluded that these objects are considered to macrophages, where the lysosomes with the absorbed fluorescent material are glowing. Dimensions of clusters of fluorescent cells and the number of the objects with a glow in them has increased up to 2 weeks after implantation, followed by a progressive decline in sizes and number.
Conclusion. AMMSCBM introduced by this method is partially absorbed by macrophages. At destruction of the structures created from AMMSCBM, detritus is also phagocytized by macrophages. In any case these macrophages appear in the lymphoid regional nodules, where initiation of immunity reactions against DNA and GFP protein isn't excluded.

Keywords: multipotent mesenchymal stromal cells, lymph nodes, macrophages, macrophage fluorescence
p. 526-532 of the original issue
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Address for correspondence:
630090, Rossiyskaya Federatsii,
g. Novosibirsk,
pr. akad. Lavrenteva, d. 8,
Institut himicheskoy biologii
i fundamentalnoy meditsinyi SO RAN,
tel.mob.: 7-913-753-07-67,
e-mail: imai@mail.ru.
Maiborodin Igor Valentinovich
Information about the authors:
Maiborodin I.V. MD, professor, leading researcher of the stem cell laboratory of SBEE HPE “Institute of chemical biology and fundamental medicine” SB RAS.
Matveeva V.A. PhD, senior researcher of the stem cell laboratory of SBEE HPE “Institute of chemical biology and fundamental medicine” SB RAS.
Maslov R.V. A post-graduate student of the hospital periodontics chair of the stomatological faculty of SBEE HPE “Moscow State Medical-Stomatological University named after A.I. Evdokimov”.
Onoprienko N.V. PhD, a senior lecturer of the obstetrics and gynecology chair of SBEE HPE “Novosibirsk State Medical University”
Kuznetsova I.V. PhD, a researcher of the stem cell laboratory of SBEE “Institute of chemical biology and fundamental medicine” SB RAS.
Chastikin G.A. PhD, applicant for Doctor’s degree of the stem cell laboratory of SBEE “Institute of chemical biology and fundamental medicine” SB RAS.
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