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Khirurgii

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Year 2021 Vol. 29 No 5

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

A.-M. YEROFEYEVA, I. ZHAVARANAK, O. ANTIPOVA, N. SCHASTNAYA, I. SIAMIONIK, S. RJABCEVA, A. MOLCHANOVA

ASSESMENT OF ANTI-NOCICEPTIVE ACTIONS OF ADIPOSE-DERIVED MESEMCHYMAL STEM CELLS IN EXPERIMENTAL PERIPHERAL NEUROPATHIC PAIN

Institute of Physiology NAS of Belarus, Minsk,
The Republic of Belarus

Objective. To estimate an anti-nociceptive and regenerative potential of adipose-derived mesenchymal stem cells in experimental post-traumatic neuropathy in rats.
Methods. Neuropathic pain was induced by axotomy technique in rat left hind paw (Wistar rats (n=113)). The respective group of subjects received ADMSCs dose of 1×106 cells/kg and 2×106 cells/kg into the site of sciatic nerve injury at 2 regimens: single (7th day post-surgery) and twice (7th and 14th day post-surgery). Nociceptive responses, as well as histological changes of sciatic nerve and perineural tissue were assessed in dynamics.
Results. Sciatic nerve axotomy led to a significant increase of mechanical nociceptive sensitivity of ipsilateral hind paw by 7th day, as well as to fibrotic changes of peri- and epineural areas of damaged nerve fibers and to denervation of surrounding muscle tissue and fascia. Local administration of ADMSCs effectively abolished mechanical hyperalgesia by 14th day after first injection at all regimens tested. Among tested regimens, the most pronounced anti-nociceptive and regenerative effects were induced by single injection of ADMSCs (1×106 cells/kg). As the dose and frequency of ADMSCs administration elevated, their reparative and anti-inflammatory properties reduced.
Conclusion. Obtained results testify anti-nociceptive potential of ADMSCs and feasibility of its further investigation on the experimental models of neuropathy.

Keywords: mesenchymal stem cells, adipose tissue, neuropathy, pain, sciatic nerve, hyperalgesia
p. 527-534 of the original issue
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Address for correspondence:
220072, Republic of Belarus,
Minsk, Akademicheskaya Str., 28,
Institute of Physiology NAS of Belarus,
the Laboratory for Modulation of Body Functions,
e-mail: amyerofeyeva@zoho.eu,
Yerofeyeva Anna-Maria V.
Information about the authors:
Yerofeyeva Anna-Maria V., Post-Graduate Student, Junior Researcher of the Laboratory for Modulation of Body Functions, Institute of Physiology NAS of Belarus, Minsk, Republic of Belarus.
https://orcid.org/0000-0002-9407-9295
Zhavaranak Irina P., PhD, Senior Researcher of the Laboratory for Modulation of Body Functions, Institute of Physiology NAS of Belarus, Minsk, Republic of Belarus.
http://orcid.org/0000-0001-9982-0719
Antipova Olga A., Researcher of the Laboratory for Modulation of Body Functions, Institute of Physiology NAS of Belarus, Minsk, Republic of Belarus.
http://orcid.org/0000-0002-5418-0874
Schastnaya Nadezhda I., Researcher of the Laboratory for Modulation of Body Functions, Institute of Physiology NAS of Belarus, Minsk, Republic of Belarus.
http://orcid.org/0000-0003-1786-5185
Siamionik Irina A., PhD, Senior Researcher of the Center for Light and Electron Microscopy, Institute of Physiology NAS of Belarus, Minsk, Republic of Belarus.
https://orcid.org/0000-0002-7520-1945
Rjabceva Svetlana N., PhD, Head of the Laboratory, Leading Researcher of the Center for Light and Electron Microscopy, Institute of Physiology NAS of Belarus, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-5960-3656
Molchanova Alla Yu., PhD, Head of the Laboratory, Leading Researcher, the Laboratory for Modulation of Body Functions, Institute of Physiology NAS of Belarus, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-5053-6602
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