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Khirurgii

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Year 2013 Vol. 21 No 4

EXPERIMENTAL SURGERY

A.V. HLUTKIN

OXYGEN-TRANSPORT FUNCTION OF BLOOD AND FREE RADICAL PROCESSES AT EXPERIMENTAL MODELING OF THE THERMAL BURN

EE “Grodno State Medical University”,
The Republic of Belarus

Objectives. To evaluate oxygen-transport function of the blood and free radical processes after thermal skin injury in an infant rat model.
Methods. The study was conducted on outbred albino infant rats (55-65 g weight, 30 days age, n=54). Thermal injury of the skin was modeled by hot liquid (water) impact at temperature of 99-100°C using a specially designed device within 10 seconds (the zone of injury was about 8-9% of the body surface). The blood gas parameters as well as hemoglobin affinity for oxygen were determined according to p50 (pO2 at 50% saturation of hemoglobin by oxygen). The sampling of tissues (lung, liver, kidney and heart) was performed to study lipid peroxidation (conjugated dienes, malondialdehyde) and antioxidant protection (α-tocopherol, catalase) in the tissues too. Determination of nitrate/nitrite concentration in the blood plasma was carried out using Griess reagent.
Results. Thermal injury in rats (30 days) leads to hypoxia based on the symptoms of metabolic acidosis and reduction of the pO2 and SO2 values as well as elevation of hemoglobin affinity for oxygen within the first 14 days and then return them to the primary values. In this case the oxidative stress has developed and its manifestation reduced to the 21st days. Also one observes an increase of nitrate/nitrite concentration, reflecting the dysfunction of L-arginine-NO system, causing changes of blood oxygen binding properties and forming prooxidant-antioxidant imbalance.
Conclusions. The conducted studies testify to an important role of the oxygen-dependent processes in the thermal injury pathogenesis which is necessary to take into consideration in developing of appropriate measures to eliminate this pathology.

Keywords: thermal burn, oxygen, blood, radical, antioxidant, nitric oxide
p. 16 – 24 of the original issue
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Address for correspondence:
210009, Respublika Belarus', g. Grodno, ul. Gor'kogo, d. 80, UO «Grodnenskii gosudarstvennyi meditsinskii universitet», kafedra detskoi khirurgii,
e-mail: glutkinalex@mail.ru,
Glutkin Aleksandr Viktorovich
Information about the authors:
Hlutkin A.V. A post-graduate student of the pediatric surgery chair of EE “Grodno State Medical University”.
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