Novosti
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This journal is indexed in Scopus |
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Year 2012 Vol. 20 No 4
EXPERIMENTAL SURGERY
YU.M. GAIN, E.P. KISSELEVA, S.V. SHACHRAI
SUBSTANTIATION OF COMPLEX TRANSPLANT APPLICATION ON THE BASIS OF AMNIOTIC MEMBRANE AND MESENCHYMAL STEM CELLS FROM THE ADIPOSE TISSUE TO RESTORE THE CUTANEOUS INTEGRITY
State Educational Establishment “Belarusian Medical Academy of Postgraduate Education”, Minsk
The Republic of Belarus
Objectives. To develop and substantiate the restoration technology of cutaneous integuments’ integrity by means of the complex transplant consisting of the amniotic membrane and the autologous mesenchymal stem cells from the adipose tissue.
Methods. From 27 laboratory animals two experimental groups (the group with application of amniotic membrane and the autologous mesenchymal stem cells from the adipose tissue, and also the control group with spontaneous regeneration of wound defect) have been formed. The area of the wound surfaces was defined on 0, 10 and 20 days after transplantation. The skin biopsy material of all animal experimental groups was examined on the 20 day after the procedure. The first result of the clinical application of the developed restoration technique of the integumentary tissues wound defect in the conditions of a chronic arterial tissue ischemia is presented.
Results. It has been found out that allogenic amniotic membrane is a universal matrix for generating a complex skin transplant with mesenchymal stem cells from the adipose tissue. It serves a universal cell frame and matrix to transfer mesenchymal stem cells from the adipose tissue providing good conditions for the growth, differentiation and implantation of the cells coated on it.
In the experiment a reliably higher rate of the wound surface regeneration was observed in the animals with the transplant application in comparison with the control group. The first experience of a clinical application of the developed technique has shown a high rate of restoration of integrity of integuments’ defects in the conditions of a chronic arterial ischemia of tissues.
Conclusions. The use of a complex transplant from amniotic membrane and mesenchymal stem cells from adipose tissue has shown the possibility of high-grade restoration of integrity of the integument both in the laboratory animals and in humans.
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210013, Respublika Belarus Minsk, ul. P. Brovki, d. 3, kor. 3,GUO «Belorusskaya meditsinskaya akademiya poslediplomnogo obrazovaniya»,
e-mail: nayka@belmapo.by,
Gain Yuriy Mihaylovich
Gain YU.M., Doctor of medical sciences, Professor, Vice-Rector on the Research Activity, Professor of the Emergency Surgery Chair of State Educational Establishment “Belarusian Medical Academy of Postgraduate Education”
Kiseleva E.P., Post-graduate Student of the Emergency Surgery Chair of State Educational Establishment “Belarusian Medical Academy of Postgraduate Education”
Shachrai S.V., Candidate of Medical Sciences, Associate Professor of the Emergency Surgery Chair of State Educational Establishment “Belarusian Medical Academy of Postgraduate Education”.
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