Novosti
|
This journal is indexed in Scopus |
|---|
Year 2023 Vol. 31 No 4
SCIENTIFIC PUBLICATIONS
H.A. POPEL, I.A. MAISEYENKA, R.R. ZHMAILIK, M.G. KALIADKA, YU. P. OSTROVSKY
EVALUATION OF THE COAGULATION HEMOSTASIS IN ANIMALS USING ROTATIONAL THROMBOELASTOMETRY AFTER ABDOMINAL AORTIC REPLACEMENT
Republican Scientific and Practical Center of Cardiology, Minsk,
Republic of Belarus
Objective. To study changes in coagulation hemostatic system of animals after abdominal aortic replacement.
Methods. The study was conducted on female white pigs (n = 12). The animals were divided into 2 groups: group 1 – with implantation of a synthetic vascular graft (SG) made of Dacron coated with modified gelatin, group 2 – with implantation of a biological vascular graft (BG) made of bovine xenopericardium. In the EXTEM, INTEM, FIBTEM and APTEM tests, the following parameters were analyzed: CT (clotting time), CFT (clot formation time), angle α, A10, A20, MCF (maximum clot firmness), ML (maximal lysis). Additionally, coagulation index, maximum clot elasticity and thrombodynamic potential index were calculated.
Results. On 1st day after operation, a slight increase in CT relative to the upper limit of the reference interval was revealed in the group of animals with biological vascular graft in INTEM, FIBTEM and APTEM tests, as well as intergroup statistically significant differences in CT values in the FIBTEM test (Me 56.0 (52.0-58.5) s and Me 49.0 (46.0-49.8) s in the BG and SG group, respectively, p = 0.029). In 14 days after surgery, animals with biological vascular graft, compared with the group of animals with a synthetic vascular graft, had higher median CT values in the EXTEM (∆ CT = 17.5 s; p = 0.041), INTEM (∆ CT = 69.0 s; p = 0.004) and APTEM (∆ CT = 9.5 c; p = 0.025) tests, as well as higher values of the clot formation time in the INTEM, FIBTEM, APTEM tests while maintaining the values of the remaining parameters within the reference intervals. By the 21st day after surgery, in the group of animals with biological graft, compared with the group of animals with synthetic graft, higher values of CT were also detected in the INTEM test: Me 137.0 (127.0-147.8) s and Me 112.0 (107.5-122.5) s respectively, (p = 0.037). At the same time, statistically significant differences in the values of CFT in the APTEM test were revealed: in the group with biological graft – Me 55.0 (51.3-60.5) s, in the group with synthetic graft – Me 41.5 (38.5-43.8) s (p = 0.004).
Conclusion. Higher values of clot formation time in the EXTEM and INTEM tests, while maintaining the values of other parameters within the boundaries of the reference intervals, indicate a slower onset of fibrin formation and, accordingly, lower thrombogenicity of a biological vascular graft, compared to a synthetic vascular graft.
- Carvalho M, Rodrigues A, Gomes M, Carrilho A, Nunes AR, Orfão R, Alves Â, Aguiar J, Campos M. Interventional Algorithms for the Control of Coagulopathic Bleeding in Surgical, Trauma, and Postpartum Settings: Recommendations From the Share Network Group. Clin Appl Thromb Hemost. 2016 Mar;22(2):121-37. doi: 10.1177/1076029614559773
- Hoareau GL, Barthélemy A, Goy-Thollot I, Pouzot-Nevoret C, Beyer CA, Walker LE, Stewart IJ, Grayson JK. Reference intervals for and the effects of sample handling and sex on rotational thromboelastometry in healthy adult pigs. J Am Assoc Lab Anim Sci. 2020 May 1;59(3):322-27. doi: 10.30802/AALAS-JAALAS-19-000095
- Münster AM, Olsen AK, Bladbjerg EM. Usefulness of human coagulation and fibrinolysis assays in domestic pigs. Comp Med. 2002 Feb;52(1):39-43. https://www.ingentaconnect.com/content/aalas/cm/2002/00000052/00000001/art00005?crawler=true&mimetype=application/pdf
- Frith D, Cohen MJ, Brohi K. Animal models of trauma-induced coagulopathy. Thromb Res. 2012 May;129(5):551-56. doi: 10.1016/j.thromres.2011.11.053
- Sunnersjö L, Lindström H, Schött U, Törnquist N, Kander T. The precision of ROTEM EXTEM is decreased in hypocoagulable blood: a prospective observational study. Thromb J. 2023 Mar 2;21(1):23. doi: 10.1186/s12959-023-00468-5
- Goggs R, Borrelli A, Brainard BM, Chan DL, de Laforcade A, Goy-Thollot I, Jandrey KE, Kristensen AT, Kutter A, Marschner CB, Rannou B, Sigrist N, Wagg C. Multicenter in vitro thromboelastography and thromboelastometry standardization. J Vet Emerg Crit Care (San Antonio). 2018 May;28(3):201-12. doi: 10.1111/vec.12710
- Zentai C, Solomon C, van der Meijden PE, Spronk HM, Schnabel J, Rossaint R, Grottke O. Effects of Fibrinogen Concentrate on Thrombin Generation, Thromboelastometry Parameters, and Laboratory Coagulation Testing in a 24-Hour Porcine Trauma Model. Clin Appl Thromb Hemost. 2016 Nov;22(8):749-59. doi: 10.1177/1076029615584662
- Ozarda Y, Sikaris K, Streichert T, Macri J; IFCC Committee on Reference intervals and Decision Limits (C-RIDL). Distinguishing reference intervals and clinical decision limits – A review by the IFCC Committee on Reference Intervals and Decision Limits. Crit Rev Clin Lab Sci. 2018 Sep;55(6):420-31. doi: 10.1080/10408363.2018.1482256
- Friedrichs KR, Harr KE, Freeman KP, Szladovits B, Walton RM, Barnhart KF, Blanco-Chavez J; American Society for Veterinary Clinical Pathology. ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics. Vet Clin Pathol. 2012 Dec;41(4):441-53. doi: 10.1111/vcp.12006
- Hanel RM, Chan DL, Conner B, Gauthier V, Holowaychuk M, Istvan S, Walker JM, Wood D, Goggs R, Wiinberg B. Systematic evaluation of evidence on veterinary viscoelastic testing part 4: Definitions and data reporting. J Vet Emerg Crit Care (San Antonio). 2014 Jan-Feb;24(1):47-56. doi: 10.1111/vec.12145
- Kessler U, Grau T, Gronchi F, Berger S, Brandt S, Bracht H, Marcucci C, Zachariou Z, Jakob SM. Comparison of porcine and human coagulation by thrombelastometry. Thromb Res. 2011 Nov;128(5):477-82. doi: 10.1016/j.thromres.2011.03.013
- Velik-Salchner C, Schnürer C, Fries D, Müssigang PR, Moser PL, Streif W, Kolbitsch C, Lorenz IH. Normal values for thrombelastography (ROTEM) and selected coagulation parameters in porcine blood. Thromb Res. 2006;117(5):597-602. doi: 10.1016/j.thromres.2005.05.015
- Lechner R, Helm M, Müller M, Wille T, Riesner HJ, Friemert B. In-vitro study of species-specific coagulation differences in animals and humans using rotational thromboelastometry (ROTEM). J R Army Med Corps. 2019 Oct;165(5):356-59. doi: 10.1136/jramc-2018-001092
- Nagler M, Kathriner S, Bachmann LM, Wuillemin WA. Impact of changes in haematocrit level and platelet count on thromboelastometry parameters. Thromb Res. 2013 Mar;131(3):249-53. doi: 10.1016/j.thromres.2013.01.009
220036, Republic of Belarus,
Minsk, R. Luxemburg Str. 110B,
Republican Scientific
and Practical Center «Cardiology»,
Vascular Surgery Research Laboratory,
tel.: +375 17 201-28-08,
e-mail: hpopel@mail.ru,
Popel Henadzi A.
Popel Henadzi A., PhD, Associate Professor, Head of Vascular Surgery Research Laboratory, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0002-1140-0806
Maiseyenka Ivan A.,Intern at Cardiac Surgery Department No. 1, Researcher at Vascular Surgery Research Laboratory, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-5403-7464
Zhmailik Ruslan R., Cardiac Surgeon at Cardiac Surgery Department No. 1, Researcher at Vascular Surgery Research Laboratory, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-6342-3527
Kaliadka Marina G., PhD, Head of Clinical Diagnostic Laboratory, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-6357-2143
Ostrovsky Yury P., MD, Professor, Academician of NAS of Belarus and RAS, Vice-Director for Innovative Development and High Technologies, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-6826-3200
Article in the .pdf format
