Novosti
Khirurgii

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Year 2023 Vol. 31 No 1

SCIENTIFIC PUBLICATIONS

O.A. KUDELICH 1, G.G. KANDRATSENCA 1, M.P. POTAPNEV 2

APPLICATION OF PLATELET-RICH PLASMA IN SEVERE ACUTE EXPERIMENTAL PANCREATITIS

Belarusian State Medical University 1,
Republican Research & Production Center for Transfusiology & Medical Biotechnologies 2, Minsk,
Republic of Belarus

Objective. To determine the effect of regional application of platelet-rich plasma on the systemic manifestations of severe acute experimental pancreatitis in rats.
Methods. This experimental study was carried out on 42 adults Wistar rats. Acute experimental pancreatitis (AEP) was induced by the introduction of a 0.3 ml of 5% solution of nonionic polyethylene glycol octylphenol ether detergent into the caudal part of the pancreas. The animals were randomly divided into 4 groups: Group I (n=6) - intact animals, Group II (control group) (n=12) – rats with AEP without treatment, Group III (n=12) - rats with AEP treated: anesthesia + infusions of 0.9% sodium chloride solution (saline), Group IV (n=12) - rats with AEP treated: anesthesia + infusions of saline + regional application of platelet-rich plasma (PRP). Animals were taken out of the experiment by euthanasia on the 3rd and 7th day. The hematological parameters, markers of systemic manifestation of the pathological process (pancreatic amylase, ASAT, ALAT, urea, creatinine), markers of endogenous intoxication (lipid peroxidation activity, nitric oxide level), markers of systemic inflammatory response (C-reactive protein, tumor necrosis factor-alpha, interleukin-6) have been evaluated.
Results. The application of PRP in the early stages of severe AEP favorably affects the change of the number and size of platelets, helps to reduce the content of free radical oxidation products, enzymes and endogenous intoxication elements, which are key links in the pathogenesis of severe acute pancreatitis. The cytoprotective effect of using PRP in the early stages of AEP on the prevention of cytolysis of hepatic and other body cells was revealed.
Conclusion. Experimental studies have revealed the ability of regional application of PRP in the early stages of AEP in rats to have a systemic positive effect, which allows considering it as a promising agent for pathogenetic treatment.

Keywords: severe acute pancreatitis, plasma, soluble platelet factors, cell therapy, PRP, biological product
p. 5-15 of the original issue
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Address for correspondence:
220116, Republic of Belarus,
Minsk, Dzerzhinsky Ave. 83,
Belarusian State Medical University,
Department of Surgery and Transplantation,
tel. mob.: +375 29 1280783,
e-mail: kudelichsurg@gmail.com,
Kudelich Oleg Arkadievich
Information about the authors:
Kudelich Oleg A., PhD, Associate Professor of the Department of Surgery and Transplantology, Belarusian State Medical University, Minsk, Republic of Belarus.
https://orcid.org/0000-0003-0569-3427
Kondratenko Gennady G., MD, Professor, Department of Surgery and Transplantology, Belarusian State Medical University, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-5295-1068
Potapnev Mikhail P., MD, Head of the Department of Cellular Biotechnologies and Medical Biotechnologies, Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies, Ministry of Health of the Republic of Belarus, Minsk, Republic of Belarus.
https://orcid.org/0000-0002-6805-1782
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