Year 2021 Vol. 29 No 5

GENERAL & SPECIAL SURGERY

N.I. TROITSKAYA, K.G. SHAPOVALOV, V.A. MUDROV

THE PREVALENCE OF GENETIC POLYMORPHISMS FOR REGULATION OF VASCULAR TONE, PLATELET RECEPTORS, VASCULAR WALL REMODELING AND PROTHROMBOTIC FACTORS IN DIABETIC FOOT SYNDROME

Chita State Medical Academy, Chita,
The Russian Federation

Objective. To analyze the frequency of occurrence of eNOS 786C>T, END1 Lys198Ash, ITGB3 1565T>C (Leu33Pro), F5 1691G>A, F2 20210G>A, MMP9 8202A>G, MTHFRA 1298 C,VEGFA 634C>G gene polymorphisms in diabetic foot syndrome.
Methods. The study included patients (n=198) with uncomplicated diabetes mellitus and patients (n=199) with the development of diabetic foot syndrome. Genotyping of the studied gene polymorphisms was performed by the polymerase chain reaction method.
Results. In patients with uncomplicated diabetes mellitus and in patients with diabetic foot, no significant differences were found in the frequency of occurrence of polymorphisms 786C>T of the eNOS3 gene, Lys198Ash of the END1 gene, 1691G>A of the F5 gene, 20210G>A of the F2 gene, 8202A>G of the MMP9 gene, 634C> G of the VEGFA gene and their allelic variants. Associative relationship between the polymorphisms of the NOS 786C>T, END1 Lys198Ash, F5 1691G>A, F2 20210G>A, MMP9 8202A>G, VEGFA 634C>G genes and the development of diabetic foot syndrome was not found. With the development of a diabetic foot, a heterozygous variant of the 1565T polymorphism of the ITGB3 gene was more common than in uncomplicated diabetes mellitus (37.7% and 28.3%, respectively (χ²=6.243, p=0.045)). The risk of developing a diabetic foot with the carriage of this polymorphism is 1.5 times higher than with other variants of gene polymorphism ((OR=1.534 (95% Cl 1.006 2.338), p<0.05). Polymorphism 1298 AS of the MTHFR gene was more often detected in the group of patients with uncomplicated diabetes mellitus than in the group with the development of diabetic foot (45.4% and 29.1%), (χ²=11.55, p=0.004).
Conclusion. The development of diabetic foot syndrome is associated with the carriage of the heterozygous 1565T polymorphism of the ITGB3 gene, in which the likelihood of developing this complication increases 1.5 fold. In diabetes mellitus without complications, a more frequent carriage of the heterozygous polymorphism 1298 AC of the MTHFR gene was revealed, which may have a protective effect against the development of diabetic foot syndrome.

Keywords: diabetic foot, gene polymorphism, vascular tone, platelet receptors, remodeling, prothrombotic factors
p. 549-557 of the original issue
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Address for correspondence:
672000, Russian Federation,
Chita, Gorkii Str., 39,
Chita State Medical Academy,
Department of Hospital Surgery,
tel.: +7 924 38045 38,
e-mail: troicachita@mail.ru,
Troitskaya Natalia I.
Information about the authors:
Troitskaya Natalia I., PhD, Assistant of the Department of Hospital Surgery, Chita State Medical Academy, Chita, Russian Federation.
https://orcid.org/0000-0002-8973-753X
Shapovalov Konstantin G., MD, Professor, Head of the Department of Anesthesiology, Resuscitation and Intensive Care, Chita State Medical Academy, Chita, Russian Federation.
https://orcid.org/ 0000-0002-3485-5176
Mudrov Viktor A., PhD, Associate Professor of the Department of Obstetrics and Gynecology of the Medical and Dental Faculties, Chita State Medical Academy, Chita, Russian Federation.
https://orcid.org/0000-0002-5961-5400
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