Novosti
Khirurgii

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Year 2019 Vol. 27 No 5

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

B.E. TULEUBAEV, D.A. SAGINOVA, A.M. SAGINOV, E.T. TASHMETOV, A.A. KOSHANOVA, A.M. BELYAYEV

ANTIBIOTIC-IMPREGNATED ALLOGRAFT: MICROBIOLOGICAL COMPARATIVE ANALYSIS

Karaganda State Medical University, Karaganda,
The Republic of Kazakhstan

Objective. To develop a method of the femoral head allograft impregnation, prepared according to the Marburg system of the bone bank, with antibiotics.
Methods. In the experiment the allografts of the femoral heads, prepared according to the Marburg system of the bone bank, were impregnated with gentamicin. Four groups were formed depending on the impregnation parameter. In the I and II groups, a whole femoral head was used, in the III and IV – perforated. In groups I and III, antibiotic impregnation was carried out simultaneously with the heat-treated femoral head and groups II and IV after heat treatment. In the control group, the whole bone allograft was subjected to standard heat treatment without its impregnation with antibiotic. From each group, 4 bone fragments were cut from a certain level of depth (cortical, subcortical, and central). The antibiotic release was evaluated by agar diffusion test against S. aureus after 24 hours.
Results. The obtained results of the study indicate the presence of antimicrobial activity in all groups except the control group. However, in the II and IV group, the inhibition zone was higher compared with the I and III groups (p <0.05). The antimicrobial activity of gentamicin in the groups where heat treatment was carried out together with the antibiotic (I, III groups) was lower than in the groups by soaking the allografts in solution with the antibiotic (II, IV groups) (p <0.05). In the control, group V, continuous growth of S. aureus was observed.
Conclusions. Allograft impregnated with antibiotic, prepared according to the Marburg system of the bone bank, provides the inhibition of growth of Staphylococcus aureusin vitro. Perforation of bone allograft according to the developed method allows increasing the antibiotic soaking on average by 10%.

Keywords: chronic osteomyelitis, bone infection, bone allograft, S. aureus, antibiotics, local transport
p. 489-495 of the original issue
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Address for correspondence:
100000, The Republic of Kazakhstan,
Karaganda, Gogol Str., 40,
Medical University of Karaganda,
Department of Surgical Diseases ¹2.
Tel. +7 701 599 8758,
e-mail: saginova@kgmu.kz,
Dina A. Saginova
Information about the authors:
Tuleubayev Berik E., MD, Professor of the Department of Surgical Diseases ¹2, Medical University of Karaganda, Karaganda, Republic of Kazakhstan.
https://orcid.org/0000-0002-9640-2463
Saginova Dina A., PhD, Assistant of the Professor of the Department of Surgical Diseases ¹2, Medical University of Karaganda, Karaganda, Republic of Kazakhstan.
http://orcid.org/0000-0001-9551-5354
Saginov Azim M., PhD, Associate Professor of the Department of Surgical Diseases ¹2, Medical University of Karaganda, Karaganda, Republic of Kazakhstan.
https://orcid.org/0000-0002-0411-8693
Tashmetov Elyarbek R., Applicant for Master’s Degree, Medical University of Karaganda, Karaganda, Republic of Kazakhstan.
http://orcid.org/0000-0002-2614-4710
Koshanova Amina A., Applicant for Doctor’s Degree, Assistant of the Department of Surgical Diseases ¹2, Medical University of Karaganda, Karaganda, Republic of Kazakhstan.
https://orcid.org/0000-0001-8620-2196
Belyayev Andrey M., PhD, Associate Professor of the Department of Clinical Immunology, Allergology, Microbiology, Medical University of Karaganda, Karaganda, Republic of Kazakhstan.
http://orcid.org/0000-0002-1527-6080
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