Year 2016 Vol. 24 No 6




SBEE HPE "Kursk State Medical University"1,
RBME "Tim Central Regional Hospital"2,
The Russian Federation

Objectives. To substantiate the possibility of application of an immobilized form of benzalkonium chloride in the treatment of experimental purulent wounds.
Methods. The experiment was carried out on Vistar male rats (n=180). Animals were divided into three groups, 60 animals per each. In the control group the topical treatment of wounds was performed using the ointment "Levomekol", while in the test group – using an immobilized form of benzalkonium chloride of the following composition: benzalkonium chloride – 0,02; metronidazolum – 1,0; polyethylene oxide M.w. 400 – 80,0; polyethylene oxide M.w. 1500 – 20,0. The dynamics of wound healing was studied by means of planimetric, bacteriological and histological methods.
Results. While studying the spectrum of antimicrobial action the designed immobilized form of benzalkonium chloride showed the best results against the test strains Staphylococcus aureus ATCC 6538-P, Bacillus cereus ATCC 10702 and Candida albicans ATCC 885-653 by comparing with the control. Planimetric study showed that the percentage reduction of wound area in the experimental group was greater than in control on the 5th, 8th and 15th day of observation, while the rate of healing in the experimental group was reliably 1,63 fold higher (p≤0,05) compared with the control. In the experimental group of animals the microbial contamination of wounds was 2,2 and 1,8 fold less than in the wounds of the control group on the 5th and the 8th day of treatment, respectively. After the histological study, it was noted that the regeneration rate was more and the reepithelialization of wound occured earlier in the experimental group than in the control, moreover by the 10th day in the experimental group the wounds had been covered by the newly formed epidermis .
Conclusion. Results of the planimetric, bacteriological and histological studies of purulent wounds in the experiment show a greater rate of recovery and expressed positive effect on wound treated with the ointment on the basis of an immobilized form of benzalkonium chloride.

Keywords: purulent wound, wound treatment, immobilized forms of benzalkonium chloride, polyethylene oxide, metronidazolum, ointment "Levomecol", healing
p. 539-545 of the original issue
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Address for correspondence:
305041, The Russian Federation,
Kursk, Karl Marx st., 3,
Kursk State Medical University,
department of operative surgery
and topographic anatomy.
Tel.: 79202675197
Grigoryan Arsen Yurevich
Information about the authors:
Gorohova A.S. Head of the surgical unit of RBME "Tim Central Regional Hospital".
Grigoryan A.Y. PhD, Ass. Professor of department of operative surgery and topographic anatomy, SBEE HPE "Kursk State Medical University".
Bezhin A.I. MD, Professor, Head of department of operative surgery and topographic anatomy, SBEE HPE "Kursk State Medical University".
Pankrusheva T.A. Dr.Sci. (Pharmacy), Professor, Head of the pharmaceutical technology department, SBEE HPE "Kursk State Medical University".
Zhilyaeva L.V. PhD, Assistant of department of microbiology, virology and immunology, SBEE HPE " Kursk State Medical University".
Mishina E.S. Assistant of department of histology, embryology and cytology, SBEE HPE "Kursk State Medical University".
Kobzareva E.V. PhD (Pharmacy), Assistant of department of disaster medicine, SBEE HPE "Kursk State Medical University".
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