Year 2015 Vol. 23 No 6




EE "Belarusian State Medical University",
The Republic of Belarus

Objectives. To evaluate the effects of local light-emitting diode phototherapy (LED) on experimental wounds.
Methods. The study was performed on 102 white inbred rats with experimental purulent (Staphylococcus aureus, Pseudomonas aeruginosa) wounds (6 subgroups with 17 animals in each). The rats in both control subgroups were not treated. Systemic antibiotic therapy was applied in the first and second subgroups of the main group; in the subgroups three and four of the main group (without antibiotic therapy) the local LED phototherapy were used sequentially: at first the antimicrobial photodynamic therapy with "Ointment Photolon" photosensitizer and emission wavelength λ1=(0,4050,002) m was applied, then the photoregulation by λ2=(0,6300,03) m was used. Conclusion about the impact of the local LED phototherapy on experimental wounds healing was made by comparing the results of visual observation, computer planimetry, histological and microbiological investigations in groups.
Results. The local application of unpolarized LED light λ1=(0,4050,002) m and medicinal agent Ointment Photolon led to the most significant reduction of the total wound microorganisms number in a short term, and the completion of the first phase of wound healing process by the seventh day of experiment. Stimulation of wound reparative processes was caused by local use of polarized LED phototherapy λ2=(0,6300,03) m after the inflammatory phase; this results in the activation of growth and maturation of granulation tissue formation as well as intensification of the epithelialization occurred from the edges of the wound.
Conclusion. The use of the local LED phototherapy with the selected parameters, taking into account the phase of experimental wound healing process resulted in the term reduction of healing no less than 5 days in vivo. The obtained results are considered to use a guide intended to develop treatment methods for designing clinical practice.

Keywords: light-emitting diode radiation, phototherapy, photosensitizer, wound process, epithelialization, granulation tissue
p. 601-611 of the original issue
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Address for correspondence:
220116, Republic of Belarus,
Minsk, pr. Dzerzhinskogo, d. 83,
UO "Belorusskiy gosudarstvennyiy
meditsinskiy universitet",
2-ya kafedra hirurgicheskih bolezney,
tel. mob. 375 29 622-57-81,
Buravsky Aleksandr Vladimirovich
Information about the authors:
Buravsky A.V. An assistant of the 2nd chair of surgical diseases of EE "Belarusian State Medical University".
Baranov E.V. PhD, an associate professor of the 2nd chair of surgical diseases of EE "Belarusian State Medical University".
Tretyak S.I. A Corresponding Member of the NAS of Belarus, Minsk, Belarus, MD, professor, a head of the 2nd chair of surgical diseases of EE "Belarusian State Medical University".
Nedzved M.K. MD, professor of the pathological anatomy chair of EE "Belarusian State Medical University"
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