Year 2013 Vol. 21 No 6




Center of New Medical Technologies of the Institute of Chemical Biology and Fundamental Medicine of Nb RAS,
The Russian Federation

Objectives. Assessment of the processes of oxidative stress and the antioxidant defense system (ADS) against the background of the stream sanitation and platelet enriched plasma (PRP) application in the treatment of chronic experimental osteomyelitis.
Methods. The study was performed on 112 albino outbred male rats. After the modeling of chronic osteomyelitis, the animals were divided into 2 control and 3 experimental groups. Treatment was not provided in the first control group. In the second control group the surgical sanitation was performed. In the experimental groups focus surgical sanitation was done. Then, in the first experimental group the stream sanitation of the damage site was performed, in the second experimental group PRP was used and in the third experimental group the combined treatment was carried out including the use of the jet sanitation and PRP. Lipid peroxidation (LPO) by the level of malondialdehyde (MDA), oxidative modification of proteins (OMP) by the content of carbonyl groups, enzymatic link of the antioxidant defense system (ADS) by the activity of superoxide dismutase (SOD) and non-enzymatic link of the ADS by the content of SH-groups were evaluated on 14th, 28th and 90th day.
Results. The dynamics of lipid peroxidation, oxidative modification of proteins as well as enzymatic and non-enzymatic units of the antioxidant defense system were studied. It has been registered that on the inflammatory process background in chronic osteomyelitis, the development of pathological activity of the free radical oxidation takes place leading to a decrease of has the tissues regenerative capacity of in the place of inflammation. Oxidative stress developed. When applying the jet sanitation and platelet rich plasma in the treatment of chronic osteomyelitis, the balanced functioning of the pro- and antioxidant defense systems is observed, preventing the development of the oxidative stress.
Conclusions. It is determined that in treatment of platelet rich plasma (PRP) and stream sanitation, a rapid cupping of oxidative stress has occurred, which contributes to the stabilization of the metabolic processes and a more favorable course of a wound healing.

Keywords: chronic osteomyelitis, lipid peroxidation, oxidative modification of proteins, antioxidant defense system
p. 17 23 of the original issue
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Address for correspondence:
630090, Rossiiskaia Federatsiia, g. Novosibirsk, pr. akad. Lavrent'eva, d. 8, Institut khimicheskoi biologii i fundamental'noi meditsiny SO RAN, Tsentr novykh meditsinskikh tekhnologii, laboratoriia stvolovoi kletki
Maiborodin Igor' Valentinovich
Information about the authors:
Maiborodin I.V. MD, professor, a leading researcher of the stem cell laboratory of the Institute of chemical biology and fundamental medicine of NB RAS.
Beregovoy E.A. PhD, an applicant for Doctors degree of the regenerative surgery laboratory of the Institute of chemical biology and fundamental medicine of NB RAS.
Kuznetsova I.V. PhD, a researcher of the stem cell laboratory of the Institute of chemical biology and fundamental medicine of NB RAS.
Shevela A.I. MD, professor, Honored physician of RF, deputy director on research of the Institute of chemical biology and fundamental medicine of NB RAS.
Barannick M.I. PhD, an applicant for Doctors degree of the stem cell laboratory of the Institute of chemical biology and fundamental medicine of NB RAS.
Maiborodina V.I. MD, a senior researcher of the stem cell laboratory of the Institute of chemical biology and fundamental medicine of NB RAS.
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