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Khirurgii

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Year 2012 Vol. 20 No 6

EXPERIMENTAL SURGERY

I.V. MAIBORODIN, V.V. MOROZOV, JA.V. NOVIKOVA, V.A. MATVEEVA, L.V. ARTEMYEVA, A.L. MATVEEV, S.V. HOMENIUK, S.V. MARCHUKOV

ACCELERATION OF DEVELOPMENT OF POSTOPERATIVE GRANULATIONS AFTER ADMINISTRATION OF MESENCHYMAL STEM CELLS NEAR THE THROMBOSED VEIN IN EXPERIMENT

Center of Modern Medical Technology of the Institute of Chemical Biology and Fundamental Medicine of Siberian branch of RAS , Novosibirsk, The Russian Federation

Objectives. To estimate the possibility of paravasal application of the autologous multipotent mesenchymal stem cells of a bone marrow origin (AMMSCBMO) for the blood flow restoration in a thrombosed vein in experiment.
Methods. The morphological results of the AMMSCBMO introduction with GFP transfected gene and DAPI cellular nuclei additionally marked with a dye near the thrombosed vein were studied by means of the fluorescence microscopy.
Results. Many vessels consisting of the specifically fluorescent cells were found out in the place of a surgical intervention performed at thrombosis modeling. The restoration of the blood flow in the thrombosed main vein is always the result of thrombolysis. The signs of AMMSCBMO embedding into the wall of thrombosed vessels haven’t been found. The recanalization of the blood clot and formation of collaterals haven’t been detected as well. At thrombosis modeling in experiment? thrombosis of the small branches occurs by introduction of thrombin and ligation of the main vein. In them restoration of blood flow occurs by involving introduced AMMSCBMO or through a blood clot recanalization or through the obliteration of thrombosed vessels and formation of new ones. Thus the restoration of the blood flow in the tissue region of the affected vein is quicker probability of development of tissue hypoxemic necroses and other complications decreases. Gradually introduced AMMSCBMO and the structures generated with their participation, are extruded by own cells an organism-recipient.
Conclusions. At introduction of AMMSCBMO in paravasal cellulose of the main thrombosed vein, they take part in restoration of the tissue blood flow in the region of the thrombosed vein, and also in development of granulations in the place of the surgical intervention performed at thrombosis modeling.

Keywords: multipotent mesenchymal stem cells, venous thrombosis, recanalization of blood clot, angiogenesis, restoration of blood flow
p. 12 -19 of the original issue
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Address for correspondence:
630090, Rossiyskaya Federatsiya, g. Novosibirsk, pr. akad. Lavrenteva, d 8, Institut himicheskoy biologii i fundamentalnoy meditsinyi SO RAN, Tsentr novyih meditsinskih tehnologiy, laboratoriya stvolovoy kletki,
e-mail: imai@mail.ru.
Maiborodin Igor Valentinovich
Information about the authors:
Maiborodin I.V. Doctor of medical sciences, professor, leading researcher of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Morozov V.V. Doctor of medical sciences, professor, leading researcher of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Novikova JA.V. Candidate of medical sciences, researcher of the invasive medical technologies laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Matveeva V.A. Candidate of biological sciences, senior researcher of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Artemyeva L.V. Leading engineer of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Matveev A.L. Post-graduate student of the molecular microbiology chair of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Homeniuk S.V. Researcher of the invasive medical technologies laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
Marchukov S.V. Post-graduate student of the stem cell laboratory of the Institute of Chemical Biology and Fundamental Medicine of RAS, Novosibirsk
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