Novosti
Khirurgii

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Year 2021 Vol. 29 No 4

GENERAL & SPECIAL SURGERY

N.I. KHRAMTSOVA, S.A. PLAKSIN, A.YU. SOTSKOV, D.N. PONOMAREV

CHARACTERISTIC OF VIABILITY OF LIPORAFT CELLS AT VARIOUS TECHNIQUES OF ITS SELECTION AND PROCESSING

Perm State Medical University named after Academician E.A. Vagner, Perm.
The Russian Federation

Objective. To determine the predictors of adipocytes and fibroblast-like cells survival in the lithographt using various techniques of lipoaspiration and adipose tissue preparation to autotraslantation.
Methods. Cellular viability was analyzed in 57 adipose tissue samples prepared by various techniques of lipoaspiration, and analysis of damage to adipocytes and fibroblast-like cells – in 73 smears after passing of adipose tissue through filters of different diameters.
Results. The average adipocyte viability in untreated lipoaspirate was 59%. Using water-jet technique, it was 65% (median 61%), syringe technique – 65% (median 74%), suction-assisted lipectomy – 55% (median 44%), p=0.18. The number of viable adipocytes depending on donor sites: thighs – 76%, waist – 67%, abdomen – 57%, buttocks – 50%, shoulders – 38%, knees – 35%. The number of intact adipocytes after 1.4 mm filter processing was 62-68%, fibroblast-like cells – 24-28%. The number of viable cells reduced with each passage. The number of intact adipocytes after 1.2 mm filter was 42-52%; of non-damaged fibroblast-like cells were 24-26% which located among connective tissue. The number of intact cells after emulsifying filter elevated up to 4-16% of adipocytes and 6-16% of fibroblast-like cells with a reduction of viable cells number with increasing of each passage. Single connective tissue fibers were determined, most content of the smear was represented by homogeneous fat.
Conclusion. The viability of adipocytes is higher when using the syringe lipoaspiration technique with sampling from the inner and outer surfaces of the thighs and lower back. For regenerative purposes, it is preferable to use emulsified fat, characterized by the destruction of adipocytes and the elimination of connective tissue fibers, keeping intact up to 16% of fibroblast-like cells. The defects of soft tissue is better to fill with washed «macro-fat» without filtration, or use a 1.4 mm cell filter. For a combination of regenerative and volumizing purposes, it is advisable to use anaerobic cell filters.

Keywords: adipocytes, regenerative medicine, adipose tissue mesenchymal stem cells, fat grafting, nanofat, lipectomy
p. 445-453 of the original issue
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Address for correspondence:
614000, Russian Federation,
Perm, Petropavlovskaya Str., 26,
Perm State Medical University Named After Academician E.A. Vagner,
the Dean’s Office of the Medical Faculty
tel.mobile: +7 909 107-12-34,
e-mail: renelve@gmail.com,
Khramtsova Natalya I.
Information about the authors:
Khramtsova Natalya I., PhD, Associate Professor of the Hospital Surgery Department, Perm State Medical University Named After Academician E.A. Vagner, Perm, Russian Federation.
http://orcid.org/0000-0001-6097-6855
Plaksin Sergey A., MD, Professor of the Surgery Department with the Course of Cardiovascular Surgery and Invasive Cardiology, Perm State Medical University Named After Academician E.A. Vagner, Perm, Russian Federation.
http://orcid.org/0000-0001-8108-1655
Sotskov Artem Yu., Student, Perm State Medical University Named After Academician E.A. Vagner, Perm, Russian Federation.
https://orcid.org/0000-0003-0225-2925
Ponomarev Danil N., Student, Perm State Medical University Named After Academician E.A. Vagner, Perm, Russian Federation.
https://orcid.org/0000-0001-5324-7515
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