Novosti
Khirurgii
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Year 2020 Vol. 28 No 5

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

DOI: https://dx.doi.org/10.18484/2305-0047.2020.5.491   |  

A.P. TRUKHAN 1, D.G. TERESHKO 2, T.A. LETKOVSKAYA 2

COMPARATIVE EVALUATION OF EFFECTIVITY OF THE INTRAPERITONEAL AND INTRAMUSCULAR ADMINISTRATION OF METHYLETHYL PYRIDINOL HYDROCHLORIDE (EMOXYPINE) IN TRAUMATIC DAMAGE OF EXTREMITY MUSCLES

432 Main Military Clinical Medical Center of the Armed Forces of the Republic of Belarus 1
Belorussian State Medical University 2, Minsk,
The Republic of Belarus

Objective. To evaluate the effectiveness of intraperitoneal and intramuscular administration of methylethylpyridinol hydrochloride (emoxypine) for traumatic injuries of the extremity muscles.
Methods. The studies were carried out on the white outbred rats (n=32). After the experimental animals were subjected to the ether anesthesia, the destruction of the striated muscles was achieved by compressing the gastrocnemius muscle in its middle third using a needle holder. 1 hour after the injury all animals underwent fasciotomy of the injured limb segment, anesthesia and emoxypine administration. The animals were divided into 4 groups (n=8 per group). To the first and third groups emoxypine was administered daily intraperitoneally once day, the second and fourth groups intramuscularly (paravulnary into the gastrocnemius muscle). The first and second groups of animals were withdrawn from the experiment on the third day, the third and fourth on the gerenth day. Subsequently, material was taken for the research, paraffin blocks were prepared and sections were made with hematoxylin and eosin staining. The assessment of morphological changes was carried out using the index of morphological changes in muscles.
Results. When assessing the severity of cell infiltration on the third day, statistically significant differences were determined in animals of the first and second groups for this indicator (U=11.5, p=0.0357). The index of morphological changes in muscles in the fourth group was 3.0 (2.5; 4.0) and significantly differed from the second (8.0 (6.0; 9.0); U=5.5, p=0, 0063). On the seventh day, the animals of the third and fourth groups also showed statistically significant differences (U=4.5, p=0.0046) in favor of intramuscular administration. Thus, on the third day with intramuscular administration of emoxypine, more pronounced cellular infiltration and less pronounced necrotic changes in the wound on the seventh day are observed, which leads to a faster wound process as a whole healing.
Conclusion. Intramuscular administration of emoxypine has a more pronounced therapeutic effect in traumatic injuries of limb muscles compared with intraperitoneal administration.

Keywords: muscle injury, antihypoxants, wound healing, reactive oxygen species, oxidative stress
p. 491-497 of the original issue
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Address for correspondence:
220034, Republic of Belarus,
Minsk, Azgur str., 4,
Belorussian State Medical University,
the Military Medical Faculty,
mobile tel.: 8-029-776-91-06,
e-mail:dmitriy-blr@mail.ru,
Tereshko Dmitry G.
Information about the authors:
Trukhan Alexey P., PhD, Associate Professor, Leading Surgeon of the Medical Unit of 432 Main Military Clinical Medical Center of the Armed Forces of the Republic of Belarus, Minsk, Republic of Belarus.
http://orcid.org/0000-0001-7422-8014.
Tereshko Dmitry G., Assistant of the Military Field Surgery of the Military Medical Faculty, the Military Medical Faculty, Belorussian State Medical University, Minsk, Republic of Belarus.
https://orcid.org/0000-0002-0235-7498.
Letkovskaya Tatyana A., PhD, Associate Professor, Head of the Pathologic Anatomy Department, Belorussian State Medical University, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-9086-2867
Contacts | ©Vitebsk State Medical University, 2007