Novosti
|
This journal is indexed in Scopus |
|---|
Year 2017 Vol. 25 No 5
GENERAL & SPECIAL SURGERY
Y.S. VINNIK, A.B. SALMINA, S.S. DUNAEVSKAYA, N.A. MALINOVSKAYA, D.A. ANTUFRIEVA
PLASMA MEMBRANE BLEBBING OF LYMPHOCYTES AS A MARKER OF ENDOTHELIAL DYSFUNCTION IN PROGRESSIVE PERIPHERAL ARTERY DISEASE OF THE LOWER EXTRIMITIES
FSBEF HE “Krasnoyarsk State Medical University named after prof. V.F. Voyno- Yasenetsky”, Krasnoyarsk,
The Russian Federation
Objectives. To determine the role of membrane blebbing of the lymphocytes as a marker of the endothelial dysfunction in the progressive clinical course of the peripheral artery disease of the legs.
Methods. The cohort prospective study was conducted enrolling 60 patients with the peripheral artery disease of the legs, with multilevel lesion of arteries, and ΙΙA stage of ischemia according to the classification of Fontaine-Pokrovsky. Based on the objective physical examination results all patients were divided into two groups; the 1st contained 30 patients with no progressive clinical course of the peripheral artery disease of the legs, the 2nd had 30 patients with a progressive clinical course. Membrane blebbing of the lymphocytes of the peripheral blood for all the patients was determined using the phase-contrast microscopy. For the objectification of the pathological process progression, Doppler ultrasound imaging of the lower limbs arteries was made to evaluate the residual vascular lumen during the first hospitalization and 6 months afterwards.
Results. Membrane blebbing of the lymphocytes of the peripheral blood is an indicator which objectively reflects the violations in the endothelium functional state. At progressive clinical course the large number of lymphocytes was revealed at the terminal blebbing state 14.9 [13.8; 19.7]%, which reflects the intensity of the pathological process. During Doppler ultrasound imaging of patients with progressive clinical course, more pronounced narrowing of the superficial femoral artery lumen is registered, which makes up -14.3 [-11.2; -17.9]%. In case of a progressive clinical course the residual vascular lumen for the posterior tibial artery was 14.5 [5.4; 21.3]% and for the anterior tibial artery it was 16.7 [11.2; 19.4]%.
Conclusions. The highest correlation coefficients with the progression of peripheral artery disease have initial (rS=0.87, p=0.001) and terminal membrane blebbing of the lymphocytes (rS=0.92, p=0.001). Complex diagnostics of the endothelium state, including membrane blebbing of the lymphocytes reflects the type of the pathological process course of the peripheral artery disease of the legs.
- Nazarova ES, Marchenko AV. Otdalennye rezul’taty konservativnogo lecheniia bol’nykh s obliteriruiushchim aterosklerozom sosudov nizhnikh konechnostei [Long-term results of conservative treatment of patients with obliterating atherosclerosis of vessels of the lower extremities]. Vestn Khirurgii im II Grekova. 2006;165(4):74-76.
- Podrezenko ES, Dunaevskaia SS. Osobennosti pokazatelei lipidnogo profilia u bol’nykh obliteriruiushchim aterosklerozom sosudov nizhnikh konechnostei [Features of lipid profile parameters in patients with obliterating atherosclerosis of vessels of lower extremities]. Vrach-Aspirant. 2014;66(5):96-100.
- Zou J, Xia Y, Yang H, Ma H, Zhang X. Hybrid endarterectomy and endovascular therapy in multilevel lower extremity arterial disease involving the femoral artery bifurcation. Int Surg. 2012 Jan-Mar;97(1):56-64. doi: 10.9738/0020-8868-97.1.56.
- Lambert MA, Belch JJ. Medical management of critical limb ischaemia: where do we stand today? J Intern Med. 2013 Oct;274(4):295-307. doi: 10.1111/joim.12102.
- Sukovatykh BS, Kniazev VV. Vliianie razlichnykh sposobov nepriamoi revaskuliarizatsii na kachestvo zhizni bol’nykh s kriticheskoi ishemiei nizhnikh konechnostei [Influence of various methods of indirect revascularization on the quality of life of patients with critical ischemia of the lower limbs]. Vestn Khirurgii im II Grekova. 2008;167(2):44-47.
- Koshkin VM, Sergeeva NA, Kuznetsov MR, Nastavsheva OD. Konservativnaia terapiia u bol’nykh khronicheskimi obliteriruiushchimi zabolevaniiami arterii nizhnikh konechnostei. Sovremennye predstavleniia [Conservative therapy in patients with chronic obliterating diseases of lower limb arteries. Contemporary representations]. Med Sovet. 2015;(8):6-9.
- Tadokoro R, Murai H, Sakai KI, Okui T, Yokota Y, Takahashi Y. Melanosome transfer to keratinocyte in the chicken embryonic skin is mediated by vesicle release associated with Rho-regulated membrane blebbing. Sci Rep. 2016 Dec 2;6:38277. doi: 10.1038/srep38277.
- Ioskevich NN, Zinchuk VV. Prooxidant-antioxidant balance in blood during the surgical treatment of obliterating arterial atherosclerosis in the lower extremities. Rocz Akad Med Bialymst. 2004;49:222-26.
660077, Russian Federation,
Krasnoyarsk, Partizana Zheleznyaka str., 1,
FSBEF HE “Krasnoyarsk State Medical University
named after prof. V.F. Voyno-Yasenetsky”,
Department of General Surgery
named after M.I. Gulman,
tel. office: 963 191-29-70,
e-mail: Vikto-potapenk@yandex.ru,
Svetlana S. Dunaevskay
Vinnik Y.S., MD, Professor, Head of the General Surgery Department named after M.I. Gulman of FSBEF HE “Krasnoyarsk State Medical University named after prof. V.F. Voyno-Yasenetsky”.
Salmina A.B., MD, Professor, Head of the Department of the Biological Chemistry with the Course of Medical, Pharmaceutical and Toxicological Chemistry of FSBEF HE “Krasnoyarsk State Medical University named after prof. V.F. Voyno-Yasenetsky”.
Dunaevskaya S.S., MD, Associate Professor, Professor of the General Surgery Department named after
M.I. Gulman of FSBEF HE “Krasnoyarsk State Medical University named after prof. V.F. Voyno-Yasenetsky”.
Malinovskaya N.A., MD, Associate Professor, Professor of the Department of the Biological Chemistry with the Course of Medical, Pharmaceutical and Toxicological Chemistry of FSBEF HE “Krasnoyarsk State Medical University named after prof. V.F. Voyno- Yasenetsky”.
Antufrieva D.A., Assistant of the General Surgery Department named after M.I.Gulman of FSBEF HE “Krasnoyarsk State Medical University named after prof. V.F. Voyno-Yasenetsky”.
Article in the .pdf format
