Year 2023 Vol. 31 No 4

SCIENTIFIC PUBLICATIONS

H.A. POPEL, I.A. MAISEYENKA, R.R. ZHMAILIK, M.G. KALIADKA, YU. P. OSTROVSKY

EVALUATION OF THE COAGULATION HEMOSTASIS IN ANIMALS USING ROTATIONAL THROMBOELASTOMETRY AFTER ABDOMINAL AORTIC REPLACEMENT

Republican Scientific and Practical Center of Cardiology, Minsk,
Republic of Belarus

Objective. To study changes in coagulation hemostatic system of animals after abdominal aortic replacement.
Methods. The study was conducted on female white pigs (n = 12). The animals were divided into 2 groups: group 1 with implantation of a synthetic vascular graft (SG) made of Dacron coated with modified gelatin, group 2 with implantation of a biological vascular graft (BG) made of bovine xenopericardium. In the EXTEM, INTEM, FIBTEM and APTEM tests, the following parameters were analyzed: CT (clotting time), CFT (clot formation time), angle α, A10, A20, MCF (maximum clot firmness), ML (maximal lysis). Additionally, coagulation index, maximum clot elasticity and thrombodynamic potential index were calculated.
Results. On 1st day after operation, a slight increase in CT relative to the upper limit of the reference interval was revealed in the group of animals with biological vascular graft in INTEM, FIBTEM and APTEM tests, as well as intergroup statistically significant differences in CT values in the FIBTEM test (Me 56.0 (52.0-58.5) s and Me 49.0 (46.0-49.8) s in the BG and SG group, respectively, p = 0.029). In 14 days after surgery, animals with biological vascular graft, compared with the group of animals with a synthetic vascular graft, had higher median CT values in the EXTEM (∆ CT = 17.5 s; p = 0.041), INTEM (∆ CT = 69.0 s; p = 0.004) and APTEM (∆ CT = 9.5 c; p = 0.025) tests, as well as higher values of the clot formation time in the INTEM, FIBTEM, APTEM tests while maintaining the values of the remaining parameters within the reference intervals. By the 21st day after surgery, in the group of animals with biological graft, compared with the group of animals with synthetic graft, higher values of CT were also detected in the INTEM test: Me 137.0 (127.0-147.8) s and Me 112.0 (107.5-122.5) s respectively, (p = 0.037). At the same time, statistically significant differences in the values of CFT in the APTEM test were revealed: in the group with biological graft Me 55.0 (51.3-60.5) s, in the group with synthetic graft Me 41.5 (38.5-43.8) s (p = 0.004).
Conclusion. Higher values of clot formation time in the EXTEM and INTEM tests, while maintaining the values of other parameters within the boundaries of the reference intervals, indicate a slower onset of fibrin formation and, accordingly, lower thrombogenicity of a biological vascular graft, compared to a synthetic vascular graft.

Keywords: rotational thromboelastometry, biological vascular graft, xenopericardium, aorta, pigs, hemostasis.
p. 265-276 of the original issue
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Address for correspondence:
220036, Republic of Belarus,
Minsk, R. Luxemburg Str. 110B,
Republican Scientific
and Practical Center Cardiology,
Vascular Surgery Research Laboratory,
tel.: +375 17 201-28-08,
e-mail: hpopel@mail.ru,
Popel Henadzi A.
Information about the authors:
Popel Henadzi A., PhD, Associate Professor, Head of Vascular Surgery Research Laboratory, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0002-1140-0806
Maiseyenka Ivan A.,Intern at Cardiac Surgery Department No. 1, Researcher at Vascular Surgery Research Laboratory, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-5403-7464
Zhmailik Ruslan R., Cardiac Surgeon at Cardiac Surgery Department No. 1, Researcher at Vascular Surgery Research Laboratory, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-6342-3527
Kaliadka Marina G., PhD, Head of Clinical Diagnostic Laboratory, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-6357-2143
Ostrovsky Yury P., MD, Professor, Academician of NAS of Belarus and RAS, Vice-Director for Innovative Development and High Technologies, Republican Scientific and Practical Center of Cardiology, Minsk, Republic of Belarus.
https://orcid.org/0000-0001-6826-3200
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