Year 2022 Vol. 30 No 6

SCIENTIFIC PUBLICATIONS

V.I. SILVISTROVICH, A.A. LYZIKOV, M.L. KAPLAN

MODIFIED POLYVINYL ALCOHOL-BASED HYDROGEL COATINGS OF PROLONGET ACTION IN THE TREATMENT OF TROPHIC ULCERS OF DIABETIC GENESIS IN EXPERIMENT

EE "Gomel State Medical University", Gomel,
Republic of Belarus

Methods. The study was performed on 240 male laboratory rats after preliminary modeling of alloxan diabetes mellitus, paw ischemia by ligation of the femoral artery, formation of a trophic ulcer by intradermal injection of 0.6 ml of 10% calcium chloride. The developed wound dressings were used in all animals, which were divided into four groups. A clinical assessment of the wound was carried out, the sampling of granulation tissue was performed on the third, seventh and 14th days. Histological material was stained with hematoxylin-eosin and trichrome MSB according to the standard method. A semi-quantitative histological evaluation was used to assess reparative processes.
Results. When applying a wound dressing without an antibacterial agent (first group), there is abundant infiltration with neutrophils, coagulation necrosis during the entire observation period. The presence of immature granulation tissue by day 14 indicates the absence of regeneration in the ulcer. The addition of gentamicin to dressing reduces inflammatory responses. Granulation tissue matures by the 7th day. Signs of epithelialization in the edges of wounds with the absence of inflammation are noted by the 14th day. With the addition of gentamicin and methyluracil to dressing, the presence of granulations was noted by the 7th day, and complete epithelization of wounds by the 14th day.
Conclusion. The addition of gentamicin and methyluracil to the composition of wound dressings statistically significantly (p<0.0001) stimulates regeneration in ulcers compared to the control group and wound dressings without gentamicin and methyluracil, and also reduces inflammatory reactions in the defect zone, stimulates the growth of granulations and their transformation into collagen (p<0.0001), which is an objective criterion for early healing.

Keywords: trophic ulcer, diabetes mellitus, wound dressing, hydrogel
p. 528-539 of the original issue
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Address for correspondence:
246000, Republic of Belarus,
Gomel, st. Lange, 5,
Gomel State Medical University,
Department of Surgical Diseases No. 1
with a Course of Cardiovascular Surgery,
tel. mob.: +375 29 6837629,
e-mail: trosko.viktoriya.89@inbox.ru,
Silvistrovich Victoria I.
Information about the authors:
Silvistrovich Victoria I., assistant of the Department of Surgical Diseases No. 1 with a course of cardiovascular surgery, Gomel State Medical University, Gomel, Republic of Belarus.
https://orcid.org/0000-0002-9968-5614
Lyzikov Aleksey An., MD, Professor, Head of the Department of Surgical Diseases No. 1 with a Course of Cardiovascular Surgery, Gomel State Medical University, Gomel, Republic of Belarus.
https://orcid.org/0000-0003-0639-121X
Kaplan Mark L., PhD Candidate of Medical Sciences, Associate Professor of the Department of Surgical Diseases No. 1 with a Course of Cardiovascular Surgery, Gomel State Medical University, Gomel, Republic of Belarus.
https://orcid.org/0000-0001-7782-3281
Osipov Boris B., PhD, Associate Professor of the Department of Surgical Diseases No. 1 with a Course of Cardiovascular Surgery, Gomel State Medical University, Gomel, Republic of Belarus.
https://orcid.org/0000-0001-9283-3680
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