Year 2022 Vol. 30 No 6

SCIENTIFIC PUBLICATIONS

T.. JARKENOV 1, S.. SAKHANOVA 1, M.N. ZHUMABAEV 1, S.T. ZAIROVA 2, M.M. MUKUSHEV 1, K.Z. NURMANOV 1

INFLUENCE OF POLYMORPHISMS OF GENES OF TUMOR NECROSIS FACTOR ALPHA, INTERLEUKIN-1β, INTERLEUKIN-6 AND INTERLEUKIN-10 ON THE DEVELOPMENT OF ACUTE PANCREATITIS IN THE KAZAKH POPULATION

West Kazakhstan Marat Ospanov Medical University 1,
Military Institute of Air Defense named T.J. Begeldinov 2, Aktobe,
Republic of Kazakhstan

Objective. To study the relationship of polymorphisms (rs1800629) of the tumor necrosis factor alpha gene, (rs16944) of the interleukin-1 gene, (rs1800795) of the interleukin-6 gene, (rs1800896) of the interleukin-10 gene with the development of acute pancreatitis in the Kazakh population.
Methods. The material for the study was DNA samples isolated from the peripheral blood of 96 unrelated patients with acute pancreatitis (44 men and 52 women) of Kazakh nationality. The assessment of the severity of the disease was based on the analysis of clinical and laboratory criteria. The control group included 108 (64 men and 44 women) unrelated Kazakh volunteers. Genotyping of polymorphisms (rs1800629) of the TNF-α -308 G/A gene, (rs16944) of the IL-1β -511 C/T gene, (rs1800795) of the IL-6 -174 G/C gene, (rs1800896) of the IL-10 -1082 gene A/G was carried out by real-time PCR.
Results. The genotypes of the studied polymorphisms in the control group were in accordance with the HardyWeinberg distribution (p>0,05). An analysis of the association of the studied genotypes with the risk of developing the disease found that the homozygous genotype -308A/A TNF-α -308 was associated with an increased risk of developing acute pancreatitis (OR=10,557, 95% CI 0,561-198,681; p=0,033). There were no significant differences in the distribution of genotypes (rs16944) of the IL-1β gene, (rs1800795) of the IL-6 gene, (rs1800896) of the IL-10 gene between patients with acute pancreatitis and control subjects (p>0,05).
Conclusion. Subjects carrying the -308A/A TNF-α, genotype have an increased risk of developing acute pancreatitis in the Kazakh population.

Keywords: cute pancreatitis, tumour necrosis factor, Interleukin-1b, Interleukin-6, Interleukin-10, genetic polymorphism
p. 510-518 of the original issue
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Address for correspondence:
030019, Republic of Kazakhstan,
Aktobe, Maresev st. 68,
West Kazakhstan Medical
University named after Marat Ospanov,
Department of Surgical Diseases No1,
grid.443411.7,
tel.: +7 7132 544297,
e-mail: timurjarkenov@zkmu.kz,
Dzharkenov Timur A.
Information about the authors:
Jarkenov Timur A., MD, Professor of the Department of Surgical Diseases No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0003-4579-6412
Sakhanova Svetlana K., MD, Associate Professor, Head of the Scientific and Practical Center, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0001-9786-6326
Zhumabaev Marat N, Ph.D., Associate Professor, Head of the Department of Surgical Diseases No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan. https://orcid.org/0000-0002-5092-6034
Zairova Saule T, PhD, Head of the Psychological Service security, Military Institute of Air Defense Named T.J. Begeldinov, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0003-3734-6884
Mukushev Manas M , PhD, Associate Professor of the Department of Surgical Diseases No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0003-1811-3276
Nurmanov Kairat Z, Assistant of the Department of Surgical Diseases No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, Republic of Kazakhstan.
https://orcid.org/0000-0003-1422-7524
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