Year 2022 Vol. 30 No 1




Vitebsk State Medical University , Vitebsk,
Minsk Scientific and Practical Center of Surgery, Transplantation and Hematology 2, Minsk,
Republic of Belarus

Objective. To study dynamics of structure and antibiotic resistance of microflora in the intensive care unit depending on the amount of used antibiotics.
Methods. 41375 isolates were studied in the intensive care unit of a multidisciplinary hospital from 2015 to early 2021. Bacteriological examination of clinical samples on basis of Republican Scientific and Practical Center Infection in Surgery has been performed. The primary treatment of results with the analysis of sensitivity of microorganisms to antibiotics was carried out by means of International Computer Program (WHONET). Antibiotic sensitivity was determined by disk-diffusion method, suspended in Mueller-Hinton broth and semi-automatic analyzer ATB Expression (BioMerieux, France). The analysis of consumption of antibacterial drugs in the intensive care unit was carried out according to data of pharmacy which operates at the medical institution where the research itself was carried out.
Results. From 2015 to 2017 the analysis of microflora composition permitted to establish the prevalence of P. aeruginosa (22,74%), Acinetobacter spp. (22,25%) and K. pneumoniae (16,11%) which occurred 1,6 folds more often than other microorganisms while in general structure of multidisciplinary hospital S. aureus was most common (20,96% and 20,05%). Since 2018 carbapenem-resistant K. pneumonia has dominated (23,01%). Tigecycline and colistat were not effective against 4,3% and 5,7% of K. pneumoniae isolates in 2019, 10,2% and 13,7% of isolates in 2020, 37,3% and 39,6% from January to May 2021 which was associated with a growth of tigecycline and colistat consumption.
Conclusion. Since 2018 prevalence of carbapenem-resistant K. pneumoniae has been observed in microflora of the intensive care unit which is associated with a growth of consumption of antibacterial drugs which caused appearance of K. pneumoniae isolates resistant to colistin and tigecycline.

Keywords: microbiota, lung microbiome, infection, antibiotics. intensive care unit, antibiotic resistance
p. 61-73 of the original issue
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Address for correspondence:
210009, Republic of Belarus,
Vitebsk, 27, Frunze Ave.,
Vitebsk State Medical University,
Department of Anesthesiology and Resuscitation,
tel. mobile: +375291460799,
Ziamko Viktoryia Y.
Information about the authors:
Ziamko Viktoryia Y., PhD, Associate Professor of the Department of Anesthesiology and Resuscitation, Vitebsk State Medical University, Vitebsk, Republic of Belarus.
Okulich Vitaly K., PhD, Associate Professor of the Department of Clinical Microbiology, Vitebsk State Medical University, Vitebsk, Republic of Belarus.
Dzyadzko Alexander M., MD, Head of the Department of Anesthesiology and Resuscitation, Minsk Scientific and Practical Center of Surgery, Transplantation and Hematology, Minsk, Republic of Belarus.
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