Year 2019 Vol. 27 No 4




Semey State Medical University, The Republic of Kazakhstan 1,
Shimane University, Faculty of Medicine, Izumi, Japan 2

Objective. Study of the azathioprine antimetabolite effect on the formation of postoperative adhesions in experiment on a rat model.
Methods. The main experiment was performed on 30 (4-5-month-old) male Wistar rats. The operation of Bloor was conducted in all animals for the initiation of the adhesive process. The rats were divided into 2 groups (15 in each): the main group (MG) animals received azathioprine in a dosage of 1 mg/100 g of weight orally 1 time per day for the first 3 days (starting from the day of surgery). The control group (CG) did not receive any medications. On the 7th day, all rats were removed from the experiment. Adhesion assessment was performed by the criteria: macroscopic (quantitative assessment of adhesions the Moreno scale, qualitative assessment - the Binda scale), histological examination. The effect of the studied method on some indicators of the immune status (leukocytes, lymphocytes, CD, CD4, CD8, PN, PI) was also studied.
Results. In CG adhesions were reported in 100% (15 rats), G 80% (12 rats). While evaluating adhesions on the Moreno and Binda scales in CG, the number of adhesions was greater 38 versus 13 (p <0.001). Also, in CG, the thickness of adhesions and their strength prevailed (p<0.001). The volume of connective tissue, the proportion of collagen fibers, the degree of vascular proliferation, fibrosis in the adhesions in OG are less pronounced (p=0.029). The immune status indicators in MG after the treatment on the 7th day were reduced slightly.
Conclusions. Immunosuppression with azathioprine significantly reduces the frequency and severity of the adhesive process in the abdominal cavity. The use of the drug in a therapeutic dose for 3 days does not lead to a significant inhibition of some of the immunity important indicators (a slight decrease in leukocytes and lymphocytes by 0.3 and 0.9, a moderate decrease in T-lymphocytes by no more than 2.0, a decrease in phagocytic activity by 5.8).

Keywords: postoperative adhesions, prevention, experiments on animals, immunosuppression, rat, azathioprine
p. 369-378 of the original issue
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Address for correspondence:
071400, The Republic of Kazakhstan,
Semey, Abay Kunanbayev Str., 103,
Semey State Medical University,
Hospital Surgery Department.
Tel.: +7 777 264-89-42,
Dauren B. Auzhanov
Information about the authors:
Auzhanov Dauren B., Applicant for Doctors Degree of Semey State Medical University, Semey, Republic of Kazakhstan.
Aymagambetov Meyrbek Zh., MD, Associate Professor, Head of the Surgery Internship Department of Semey State Medical University, Semey, Republic of Kazakhstan.
Zhagiparova Zhanar A., PhD, Head of the Department of Special Disciplines of Pavlodar Branch of Semey State Medical University, Semey, Republic of Kazakhstan.
Noso Yoshihiro, MD, PhD, Professor of the General Medicine Department of the Faculty of Medicine, Shimane University, Izumi, Japan.
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