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indexed in Scopus
Year 2018 Vol. 26 No 5
V.D. SHYSHCHUK1, S.I. REDKO1, M.N. OGIENKO2, D.V. OVECHKIN1, L.V. TOMIN1
DISTURBANCES OF REPARATIVE OSTEOGENESIS IN THE HYPOVOLEMIA AND THEIR CORRECTION IN EXPERIMENT
Sumy State University 1,
Sumy City Clinical Hospital ¹5 2, Sumy,
Objective. To study reparative osteogenesis features in a long tubular bone of rats with moderate isoosmolar dehydration and the determination of the possibility to correlate the morphofunctional changes, caused by dehydration using the drug Actovegin.
Methods. In male Wistar rats in the control (n=21) and experimental (n=21) groups, the trauma was simulated by applying a perforated defect in the middle third of the both tibia bones diaphysis. Moderate dehydration was achieved by total water deprivation for 6-7 days until 6-10% animal weight loss was reached. In the experimental group, Actovegin was administered 2 mg intramuscularly every other day. The introduction of the drug began immediately after the injury and continued until the end of the experiment. On the 3rd, 15th and 24th day, histological examination, morphometry and scanning electron microscopy was carried out to study morphological changes in rat tibial reclaims.
Results. Moderate isoîsmolar dehydration caused a disruption of cellular and tissue balance which slowed down the bone regeneration process without changing the staging of their course. As a result, a normal bone callus did not have time to form in expected terms. Actovegin stimulated the reparative bone regeneration, the ratio of tissue components of the regenerate changed, the area increased and the qualitative properties of coarse-fiber and lamellar bone tissues were improved in comparison with the indices of animals in the control group. An increase in the total area and diameter of the vessels in the regenerate was registered.
Conclusions. The use of the drug Àctovegin permitted to optimize the blood circulation in the damaged segment and to accelerate the processes of bone restructuring and maturation resulting in the formation of a full-blown bone callus.
- Hernlund E, Svedbom A, Ivergard M, Compston J, Cooper C, Stenmark J, McCloskey EV, Jönsson B, Kanis JA. Osteoporosis in the European Union: medical management, epidemiology and economic burden. A report prepared in collaboration with the International Osteoporosis Foundation (IOF) and the European Federation of Pharmaceutical Industry Associations (EFPIA). Arch Osteoporos. 2013;8:136. doi: 10.1007/s11657-013-0136-1
- Malinovskii EL, Nadyrov EA, Nikolaev VI. Optimizatsiia reparativnogo osteogeneza pri politravme. Novosti Khirurgii. 2011;19 (5):17-22. http://www.surgery.by/pdf/full_text/2011_5_3_ft.pdf (in Russ.)
- Gulevsky AK, Abakumova YS, Moiseyeva NN, Ivanov YG. Influence of cord blood fraction (below 5 kDa) in reparative processes during subchronic ulcerative gastropathy. Ulcer. 2011(2011):1-9. doi: 10.1155/2011/214124
- Stelmakh A, Abrahamovych O, Cherkas A. Highly purified calf hemodialysate (Actovegin®) may improve endothelial function by activation of proteasomes: a hypothesis explaining the possible mechanisms of action. Med Hypotheses. 2016 Oct;95:77-81. doi: 10.1016/j.mehy.2016.09.008
- Søndergard SD, Dela F, Helge JW, Larsen S. Actovegin, a non-prohibited drug increases oxidative capacity in human skeletal muscle. Eur J Sport Sci. 2016 Oct;16(7):801-7. doi: 10.1080/17461391.2015.1130750
- Lee P, Nokes L, Smith PM. No effect of intravenous Actovegin® on peak aerobic capacity. Int J Sports Med. 2012 Apr;33(4):305-9. doi: 10.1055/s-0031-1291322
- Wu SX, Cui TT, Zhao C, Pan JJ, Xu BY, Tian Y, Cui NJ. A prospective, randomized, multi-center trial to investigate Actovegin in prevention and treatment of acute oral mucositis caused by chemoradiotherapy for nasopharyngeal carcinoma. Radiother Oncol. 2010 Oct;97(1):113-18. doi: 10.1016/j.radonc.2010.08.003
- Guekht A, Skoog I, Edmundson S, Zakharov V, Korczyn AD. ARTEMIDA Trial (A Randomized Trial of Efficacy, 12 Months International Double-Blind Actovegin): A Randomized Controlled Trial to assess the efficacy of actovegin in poststroke cognitive impairment. Stroke. 2017 May;48(5):1262-1270. doi: 10.1161/STROKEAHA.116.014321
- Soboleva AD. Reaktsiia kletok i tkanei na obezvozhivanie. Novosibirsk, SSSR: Nauka; 1975. 64 p. (in Russ.)
- Korzh NA, Dedukh NV. Reparativnaia regeneratsiia kosti: sovremennyi vzgliad na problemu. Stadii regeneratsii (soobshch 1). Ortopediia, Travmatologiia i Protezirovanie. 2006;(1)77-84. (in Russ.)
- Logosha AI, Slisarenko AV, Ogienko MN, Bumeister VI, Prikhod’ko OA. Reparativnyi osteogenez trubchatykh kostei v usloviiakh narusheniia vodno-solevogo obmena. Georgian Medical News. 2013;(10):80-86. http://www.geomednews.org (in Russ.)
- Oryan A, Monazzah S, Bigham-Sadegh A. Bone injury and fracture healing biology. Biomed Environ Sci. 2015 Jan;28(1):57-71. doi: 10.3967/bes2015.006
Sumy, Sanatornaya Str., 31,
Medical Institute of Sumy State University,
Department of Orthopedics
Tel. mob.: 380 066 08 27 869,
Sergiy I. Redko
Shyshchuk Volodymir D., MD, Professor, Head of the Department of Orthopedics and Traumatology of Medical Institute, Sumy State University, Sumy, Ukraine.
Redko Sergiy I., Assistant of the Department of Orthopedics and Traumatology of Medical Institute, Sumy State University, Sumy, Ukraine.
Ogienko Maksym N., PhD, Physician, Sumy City Clinical Hospital ¹5, Sumy, Ukraine.
Ovechkin Denys V., PhD, Associate Professor of the Department of Orthopedics and Traumatology of Medical Institute, Sumy State University, Sumy, Ukraine.
Tomin Lubov V., Post-Graduate Student of the Department of Orthopedics and Traumatology of Medical Institute, Sumy State University, Sumy, Ukraine.