Year 2018 Vol. 26 No 5




Sumy State University 1,
Sumy City Clinical Hospital 5 2, Sumy,

Objective. To study reparative osteogenesis features in a long tubular bone of rats with moderate isoosmolar dehydration and the determination of the possibility to correlate the morphofunctional changes, caused by dehydration using the drug Actovegin.
Methods. In male Wistar rats in the control (n=21) and experimental (n=21) groups, the trauma was simulated by applying a perforated defect in the middle third of the both tibia bones diaphysis. Moderate dehydration was achieved by total water deprivation for 6-7 days until 6-10% animal weight loss was reached. In the experimental group, Actovegin was administered 2 mg intramuscularly every other day. The introduction of the drug began immediately after the injury and continued until the end of the experiment. On the 3rd, 15th and 24th day, histological examination, morphometry and scanning electron microscopy was carried out to study morphological changes in rat tibial reclaims.
Results. Moderate isosmolar dehydration caused a disruption of cellular and tissue balance which slowed down the bone regeneration process without changing the staging of their course. As a result, a normal bone callus did not have time to form in expected terms. Actovegin stimulated the reparative bone regeneration, the ratio of tissue components of the regenerate changed, the area increased and the qualitative properties of coarse-fiber and lamellar bone tissues were improved in comparison with the indices of animals in the control group. An increase in the total area and diameter of the vessels in the regenerate was registered.
Conclusions. The use of the drug ctovegin permitted to optimize the blood circulation in the damaged segment and to accelerate the processes of bone restructuring and maturation resulting in the formation of a full-blown bone callus.

Keywords: actovegin, dehydration, bony callus, morphometry, raster microscopy
p. 526-534 of the original issue
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Address for correspondence:
40018, Ukraine,
Sumy, Sanatornaya Str., 31,
Medical Institute of Sumy State University,
Department of Orthopedics
and Traumatology,
Tel. mob.: 380 066 08 27 869,
Sergiy I. Redko
Information about the authors:
Shyshchuk Volodymir D., MD, Professor, Head of the Department of Orthopedics and Traumatology of Medical Institute, Sumy State University, Sumy, Ukraine.
Redko Sergiy I., Assistant of the Department of Orthopedics and Traumatology of Medical Institute, Sumy State University, Sumy, Ukraine.
Ogienko Maksym N., PhD, Physician, Sumy City Clinical Hospital 5, Sumy, Ukraine.
Ovechkin Denys V., PhD, Associate Professor of the Department of Orthopedics and Traumatology of Medical Institute, Sumy State University, Sumy, Ukraine.
Tomin Lubov V., Post-Graduate Student of the Department of Orthopedics and Traumatology of Medical Institute, Sumy State University, Sumy, Ukraine.
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