This journal is
indexed in Scopus
Year 2018 Vol. 26 No 3
N.A. SHCHUDLO, T.N. VARSEGOVA, M.M. SHCHUDLO, L.I. SBRODOVA, T.A. STUPINA
INTERRELATION BETWEEN PRIMARY AND SECONDARY CONTRACTION WITH HISTOLOGICAL STRUCTURE OF FASCIOCUTANEOUS FLAP
Russian Ilizarov Scientific Center of Restorative Traumatology and Orthopedics, Kurgan,
The Russian Federation
Objective. To conduct histomorphometric assessment of the skin in fasciocutaneous flap with regard to its primary and secondary contraction degree.
Methods. The experiments were carried out on 28 rats of the Wistar line. In animals a fasciocutaneous flap was formed according to a template with the area of 18 cm2 on the basis of a superficial epigastric artery that was clipped; after 1.5 hours the flap was reperfused and re-donated. The animals were euthanized 12 days after the operation. The methods used in the study were the analysis of the leukocyte blood formula, computerized planimetry of the digital images of flaps, histomorphometry, immunohistochemistry.
Results. Right after the flap reposition in four rats, its area increased by 6-30% compared to the template and decreased by 9-34% in 24 rats. At the end of the experiment, rats were divided into 4 groups: 1 – an increase in the flap area (+ 5-19%), 2 – a decrease in primary contraction (up to -1-14%), 3 – significant primary (-22-34%) and secondary -20-25%) contraction, 4 – expressed secondary contraction (-30-59%). Defects of the epidermis associated with delayed wound healing and partial necrosis in groups 1-3 did not exceed 3%, and in group 4 varied from 8 to 41% of the area of the flap. The highest degree of epidermal hypoplasia was registered in group 1 (47% of thickness measurements), hyperplasia in group 4 (67%). In comparison with a normal skin, the numerical densities of the skin appendages and the volume density of the adipocytes of the dermis progressively decreased in groups 1-4, the severity of postnecrotic changes in the dermis and hypodermis increased.
Conclusions. The obtained results substantiate the necessity for monitoring of the primary and secondary contraction of the vascularized fasciocutaneous flaps to elaborate individually oriented therapy protocols in order to modulate the proliferative activity of epidermis and revascularization of the vascular plexus of the skin.
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640014, The Russian Federation,
Kurgan, M. Ulyanova Str., 6,
Russian Ilizarov Scientific Center
of Restorative Traumatology and Orthopedics,
Clinical and Experimental
Laboratory of Reconstructive-Plastic
Microsurgery and Hand Surgery,
Tel. office: +7-3522 45-18-12
Nathalia A. Shchudlo
Shchudlo Nathalia A., MD, Chief Researcher of the First Scientific and Clinical Department, Russian Ilizarov Scientific Center of Restorative Traumatology and Orthopedics, Kurgan, Russian Federation.
Varsegova Tatyana N., PhD, Senior Researcher of the Morphology Laboratory, Russian Ilizarov Scientific Center of Restorative Traumatology and Orthopedics, Kurgan, Russian Federation.
Shchudlo Michael M., MD, Leading Researcher of the First Scientific and Clinical Department, Russian Ilizarov Scientific Center of Restorative Traumatology and Orthopedics, Kurgan, Russian Federation.
Sbrodova Lyudmila I., PhD, Researcher of the Scientific and Clinical Laboratory of Microbiology and Immunology, Russian Ilizarov Scientific Center of Restorative Traumatology and Orthopedics, Kurgan, Russian Federation.
Stupina Tatyana A., DS, Senior Researcher of the Morphology Laboratory, Russian Ilizarov Scientific Center of Restorative Traumatology and Orthopedics, Kurgan, Russian Federation.