Year 2018 Vol. 26 No 2




Sumy State University, Medical Institute,

Objective. This preliminary study was conducted to assess the possible association of IL-8 (-251/) polymorphism with clinical course and outcome of acute pancreatitis aggravated by pancreatogenic peritonitis.
Methods. Data for the study were DNA samples, received from the leucocytes of 143 humans: 83 patients with acute pancreatitis aggravated by pancreatogenic peritonitis, 60 healthy blood donors without acute pancreatitis in the anamnesis served as controls. IL-8 (-251/) polymorphism detection was made with polymerase chain reaction with further length analysis of the restriction fragments.
Results. The analysis of frequency of allelic variants of the cytokine gene IL-8 (-251/) revealed that genotype A/T was the dominant variant (45%) among healthy blood donors. Distribution of IL-8 (-251/) polymorphism among the patients with pancreatogenic peritonitis without and after surgical treatment is characterized by dominance of genotype T/T in group after surgical treatment (<0.05). This may indicate association of genotype T/T and unfavorable clinical course of pancreatogenic peritonitis (OR>1). Among patients after surgical treatment genotype A/T was less often met in comparison with the group without it (p<0.05). This may indicate the association of genotype A/T and favorable clinical course of pancreatogenic peritonitis (OR<1). Genotype A/A was rarely registered, which may be due to regional peculiarities of the patients genotype.
Conclusions. This preliminary study suggests that the identification of genetic polymorphism of IL-8 (-251A/T)
may be informative and serve as an additional criterion to predict both the clinical course and outcome of pancreatogenic peritonitis; it may also specify indications for surgical treatment. However, the possible role of IL-8 (-251A/T) cytokine polymorphism in the outcome of pancreatogenic peritonitis requires further carefully planned cohort investigations.

Keywords: acute pancreatitis, pancreatogenic peritonitis, interleukin-8, gene polymorphism, immune response, cytokines
p. 163-168 of the original issue
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Address for correspondence:
40007, Ukraine,
Sumy, Rimsky-Korsakov Str., 2,
Sumy State University,
Department of Surgery and Oncology,
Tel. office: +380 542 64-03-18,
Chumakov Volodymyr M.
Information about the authors:
Chumakov Volodymyr N., Assistant of the Department of Surgery and Oncology, Sumy State University, Sumy, Ukraine.
Sytnik Oleksandr L., PhD, Associate Professor of the Department of Surgery and Oncology, Sumy State University, Sumy, Ukraine.
Bugaiov Volodymyr I., PhD, Associate Professor of the Department of Surgery and Oncology, Sumy State University, Sumy, Ukraine.
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