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Khirurgii

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Year 2018 Vol. 26 No 2

GENERAL & SPECIAL SURGERY

I.A. SANETS ¹, A.E. SILIN ¹, YU.I. YARETS ¹, V.V. ANICHKIN ²

GENETIC MARKERS OF VENOUS THROMBOEMBOLISM IN SURGICAL PATIENTS

The Republican Research Centre for Radiation Medicine and Human Ecology 1,
Gomel State Medical University2, Gomel
The Republic of Belarus

Objective. Òo study the occurrence of genetic markers of the venous thromboembolism (FV:G1691A, FII:G20210A, MTHFR:C677T and PAI-1:5G/4G) in patients who have previously had deep vein thrombosis and/or the pulmonary embolism as well as in surgical patients without phlebothrombosis episodes in the anamnesis.
Methods. The subjects of the study were patients (n=54) suffering from cholelithiasis. All individuals were divided into two groups: group 1 consisted of patients with deep vein thrombosis and/or pulmonary embolism in the past (n=29, 12 males and 17 females, the mean age of patients was 59.4±12.4 years). The second group was presented by patients without venous thromboembolism in the case history (n=25, 9 males and 16 females, the mean age – 54.7±8.4 years). The molecular genetic testing of four main factors associated with a predisposition to thrombotic complications (FV:G1691A, FII:G20210A, MTHFR:C677T and PAI-1:5G/4G) was performed in patients of both groups. The study was carried out using the PCR method with three different oligonucleotide primers to identify each genetic polymorphism (mutation).
Results. The incidence of Leiden mutation (FV:G1691A) in the surgical patients with the venous thromboembolism was significantly higher than in patients without phlebothrombosis episodes in the past (25% and 4%, respectively, ð=0.038).
There were no significant differences between the groups of patients with the venous thrombotic complications and in the control group patients in terms of others genetic polymorphism: FII: G20210A (ð=0.349), PAI-1: 5G/4G (ð=0.751) and MTHFR: C677T (ð=0.416).
Conclusions. The presence of the Leiden mutation of the V coagulation factor in surgical patients significantly increases the risk of thrombotic complications in the postoperative period. The presence of FII:G20210A, PAI-1:5G/4G, MTHFR:C677T genetic defects in surgical patients are not associated with the likelihood of the postoperative thrombosis development.

Keywords: venous thromboembolism, Leiden mutation, prothrombin, methylene tetrahydrofolate reductase, inhibitor of plasminogen activation
p. 155-162 of the original issue
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Address for correspondence:
246040, The Republic of Belarus,
Gomel, Ilyich Str., 290,
Republican Scientific Center
for Radiation Medicine and Human Ecology,
Surgical Unit of the Consultative Polyclinic,
Tel. office: +375-232-38 96 36,
e-mail: igor-sanets@yandex.ru,
Sanets Igor A.
Information about the authors:
Sanets Igor A., Surgeon, Surgical Unit of the Consultative Polyclinic, the Republican Scientific Center for Radiation Medicine and Human Ecology, Gomel, Republic of Belarus.
http://orcid.org/0000-0001-5777-3416
Yarets Yuliya I., PhD, Associate Professor, Head of the Clinical and Diagnostic Laboratory, the Republican Scientific Center for Radiation Medicine and Human Ecology, Gomel, Republic of Belarus.
http://orcid.org/0000-0001-8879-5079
Silin Arkadij E., PhD, Head of the Laboratory of Molecular Genetics, the Republican Scientific Center for Radiation Medicine and Human Ecology, Gomel, Republic of Belarus.
http://orcid.org/0000-0003-2387-554x
Anichkin Vladimir V., MD, Professor, Professor of the Department of Surgical Diseases ¹3 with the Course of Urology, Gomel State Medical University, Gomel, Republic of Belarus.
http://orcid.org/0000-0002-9022-5320
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