Year 2017 Vol. 25 No 6

EXPERIMENTAL SURGERY

O.V. FALKO, N.A. SHEVCHENKO, V.Y. PROKOPYUK, A.A. ROENKO, O.S. PROKOPYUK

COMPARATIVE ANALYSIS OF LIMBS TROPHIC ULCERS MODELING METHODS IN MICE

Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, Kharkov, Ukraine

Objective. To compare the existing methods of modeling an ulcerative defect as well as hemodynamics and innervation disorders, and then to develop the model of the lower limbs trophic ulcer in mice.
Methods. Trophic ulcers were simulated in male Balb/ mice. Vascular insufficiency was formed by either ligation (group 1) or electrocoagulation (group 2) of the neurovascular bundle at the site of bifurcation of the common iliac and femoral arteries and veins. The ulcerative defect was formed by intradermal injection of 0.1 ml 10% calcium chloride (groups 3, 5 and 7) and 9% acetic acid (groups 4, 6 and 8). The animals of the groups 5 and 6 were electrocoagulated prior to administration of chemical agents and the animals of the groups 7 and 8 after it. The term of ulceration, ulcer area, period of complete healing and functional status of the limb were determined.
Results. Ligation (group 1) resulted in a mummification of limb tissues and electrocoagulation (group 2) led to a temporary disruption of function without forming ulcerous surface. Dry ulcer had been revealed in the animals of the groups 3 and 5 by the 10th day; the ulcer had been completely healed by the 20th day and the 30th day, respectively. A weeping ulcer was revealed in mice of the groups 4 and 8 to day 3; its healing had been observed by the 15th and the 35th, respectively. No ulcerative defects were observed in the animals of the groups 6 and 7, there was observed necrotic injury of skin and under laying structures. Impaired functional activity of the injured limb was found in all experimental groups. In the animals of the 1st and 7th groups the limb function did not recover up to the end of the experiment.
Conclusions. Intradermal administration of acetic acid and subsequent electrocoagulation of neurovascular bundle allow forming a long-lasting non-healing weeping ulcer and limb dysfunction, which were typical for trophic ulcers. Other studied techniques can be applied when simulating various diseases, related to vascular, nervous and skin damages.

Keywords: trophic ulcer, modeling, mice, electrocoagulation, ligation, acetic acid, calcium chloride
p. 561-566 of the original issue
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Address for correspondence:
61016, Ukraine,
Kharkov, Pereyaslavskaya str., 23,
Institute for Problems of Cryobiology
and Cryomedicine of NAS of Ukraine,
Department of Cryobiology
of the Reproduction Systems,
tel. office.: +38 057 373-59-53,
e-mail: o.v.falko@gmail.com,
Oksana V. Falko
Information about the authors:
Falko O.V., PhD, Researcher of the Department of Cryobiology of the Reproduction Systems for the Institute of Problems of Cryobiology and Cryomedicine of NAS of Ukraine.
Shevchenko N.A., PhD, Senior Researcher of the Department of Cryobiology of the Reproduction Systems of the Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine.
Prokopyuk V.Y., PhD, Senior Researcher of the Department of Cryopathophysiology of the Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine.
Roenko A.A., Post-Graduate Student of the Department of Cryobiology of the Reproduction Systems of the Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine.
Prokopyuk O.S., MD, Professor, Head of the Department of Cryobiology of the Reproduction Systems of the Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine.
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