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Khirurgii

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Year 2017 Vol. 25 No 5

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

I.Y. TSYMBALYUK, A.M. MANUILOV, K.A. POPOV, A.A. BASOV

METABOLIC CORRECTION OF THE ISCHEMIA-REPERFUSIVE INJURY WITH SODIUM DICHLOROACETATE IN VASCULAR ISOLATION OF THE LIVER IN EXPERIMENT

FSBEE HE “Kuban State Medical University” of the Ministry of Health of the Russian Federation, Krasnodar,
The Russian Federation

Objectives. To study the effect of sodium dichloroacetate on the ischemia course and the development of the reperfusive syndrome as a result of the vascular liver isolation in rats.
Methods. The experimental study was performed on 110 non-linear male rats divided into 7 groups. Under the general anesthesia the laparotomy was carried out, sodium dichloroacetate has been injected intraperitoneally in the dosage of 300 mg/kg, the hepatoduodenal ligament was exposed and clamped within 10, 15 and 20 minutes. The comparison groups were composed of rats that had undergone the same manipulations except the sodium dichloroacetate injections. The control group included rats that had undergone only laparotomy. On the 15th reperfusion minute the animals were subjected to the blood and liver sampling for further laboratory studies. In the blood plasma the activity of the LDH, the AST and the ALT was determined. In erythrocytes and the liver homogenate, the activity of the glutathione peroxidase, the glutathione reductase and the content of the reduced glutathione were determined.
Results. Sodium dichloroacetate considerably improves the liver ischemia tolerance which has been confirmed by the lowering of the hepatocyte cytolysis. Thus the LDH activity in the blood plasma was 2-3.2 times lower than the indices of the control groups. The research of the glutathione metabolism against the background of correction by means of sodium dichloroacetate has also revealed the enhancement of this link in the non-specific resistance system. Besides the activity of the glutathione reductase and the glutathione concentration in erythrocytes have not decreased even during the 20-minute ischemia. In the liver homogenate the increase in the glutathione peroxidase by 1.7-2.8 times has been revealed. The concentration of the restored glutathione has decreased by the 10-minute ischemia by 30% and further has maintained on that level. Such changes can be caused by the intensive system functioning of the glutathione metabolism by local injuries which is accompanied by the more active resistance to the injuring factor.
Conclusions. The presented data demonstrate the cytoprotective effect of sodium dichloroacetate on the models of the ischemic-reperfusive liver injury as a result of its vascular isolation.

Keywords: Pringle maneuver, hepatic ischemia reperfusion injury, metabolic cytoprotection, sodium dichloroacetate, glutathione system
p. 447-453 of the original issue
References
  1. Khubutiia MSh, Chzhao AV, Dzhagraev KR, Andreitseva OI, Zhuravel’ SV, Salienko AA, i dr. Transplantatsiia pecheni kak radikal’nyi metod lecheniia konechnykh stadii zabolevanii pecheni [Liver transplantation as a radical treatment for the final stages of liver disease]. Prakt Meditsina. 2010;8(47):13-19.
  2. Akhmetzianov FSh, Idrisov MN. Sposoby rezektsii pecheni [Methods of liver resection]. Kazan Med Zhurn. 2015;96(4):563-67. doi: 10.17750/KMJ2015-563.
  3. van der Bilt JDW, Livestro DP, Borren A, van Hillegersberg R, Borel Rinkes IHM. European survey on the application of vascular clamping in liver surgery. Dig Surg. 2007;24(6):423-35. doi: 10.1159/000108325.
  4. Benzoni E, Cojutti A, Lorenzin D, Adani GL, Baccarani U, Favero A, et al. Liver resective surgery: a multivariate analysis of postoperative outcome and complication. Langenbecks Arch Surg. 2007 Jan;392(1):45-54.
  5. Shcherba AE, Kirkovskii LV, Dziadz’ko AM, Avdei EL, Minov AF, Bolonkin LS, i dr. Rezektsiia pecheni v usloviiakh gipotermicheskoi konservatsii [Liver resection under hypothermic conservation conditions]. Novosti Khirurgii. 2012;20(6):45-52.
  6. Khodosovskii MN. Korrektsiia okislitel’nykh povrezhdenii pri sindrome ishemii-reperfuzii pecheni [Correction of oxidative damage in the ischemia-reperfusion syndrome of the liver]. Zhurn GrGMU. 2016;(4):20-25.
  7. Cao L, Quan XB, Zeng WJ, Yang XO, Wang MJ. Mechanism of Hepatocyte Apoptosis. J Cell Death. 2016;9:19-29. doi: 10.4137/JCD.S39824.
  8. Li J, Li RJ, Lv GY, Liu HQ. The mechanisms and strategies to protect from hepatic ischemia-reperfusion injury. Eur Rev Med Pharmacol Sci. 2015;19(11):2036-47.
  9. Donadon M, Molinari AF, Corazzi F, Rocchi L, Zito P, Cimino M, et al. Pharmacological Modulation of Ischemic-Reperfusion Injury during Pringle Maneuver in Hepatic Surgery. A Prospective Randomized Pilot Study. World J Surg. 2016 Sep;40(9):2202-12. doi: 10.1007/s00268-016-3506-1.
  10. Bykov MI, Basov AA. Change of parameters in prooxidant-antioxidant bile system in patients with the obstruction of bile-excreting ducts. Med Vestn Severnogo Kavkaza. 2015;10(2):131-35. doi: 10.14300/mnnc.2015.10029.
  11. Knoechel TR, Tucker AD, Robinson CM, Phillips C, Taylor W, Bungay PJ, et al. Regulatory roles of the N-terminal domain based on crystal structures of human pyruvate dehydrogenase kinase 2 containing physiological and synthetic ligands. Biochemistry. 2006 Jan 17;45(2):402-15. doi: 10.1021/bi051402s.
  12. Salamon S, Podbregar E, Kubatka P, Büsselberg D, Caprnda M, Opatrilova R, et al. Glucose Metabolism in Cancer and Ischemia: Possible Therapeutic Consequences of the Warburg Effect. Nutr Cancer. 2017 Feb-Mar;69(2):177-83. doi: 10.1080/01635581.2017.1263751.
  13. Karpishchenko AI. Meditsinskie laboratornye tekhnologii [Medical laboratory technologies]: Spravochnik. S-Petersburg, RF: Intermedika; 2002. T. 2. 600 p.
  14. Kolesnichenko LS, Kulinsky VI, Sotnikova GV, Kovtun VYu. Influence of changes in glutathione concentration on body temperature and tolerance to cerebral ischemia. Biochemistry (Moscow). 2003;68(5):534-40. doi: 10.1023/A:1023903609320.
  15. Kulinskii VI, Kolesnichenko LS. Sistema glutationa I. Sintez, transport, glutationtransferazy, glutationperoksidazy [Synthesis, transport, glutathione transferase, glutathione peroxidase]. Biomed Khimiia. 2009;55(3):255-77.
Address for correspondence:
350063, Russian Federation,
Krasnodar, Sedina str., 4,
FSBEE HE “Kuban State Medical University”,
Department of Surgery №2
of the Faculty of the Advanced Training
and Retraining of Specialists,
tel. mob.: 7 928 430-07-69,
e-mail: igor_ts@inbox.ru,
Igor Y. Tsymbalyuk
Information about the authors:
Tsymbalyuk I.Y., Post-Graduate Student, Senior Laboratory Assistant of the Surgery Department №2 of the Faculty of the Advanced Training and Retraining of Specialists of FSBEE HE “Kuban State Medical University”.
Manuilov A.M., MD, Professor, Head of the Surgery Department №2 of the Faculty of the Advanced Training and Retraining of Specialists of FSBEE HE “Kuban State Medical University”.
Popov K.A., Post-Graduate Student, Assistant of the Fundamental and Clinical Biochemistry Department of FSBEE HE “Kuban State Medical University”.
Basov A.A., MD, Professor of the Fundamental and Clinical Biochemistry Department of FSBEE HE “Kuban State Medical University”.
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