Year 2016 Vol. 24 No 2




Ternopol State Medical University Named after I.Y.Gorbachevsky,

Objectives. To carry out the morphometric analysis of the skin layers affected by thermal factor in early necrectomy performance with subsequent wound closure by means of hydrogel dressings and spongy polyurethane adsorbents.
Methods. The study was conductred on mature white outbred rats (n=192), weighing 250-300 g. Due to the European Convention for the Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes, the damage conducted under general ether anaesthesia according to Regas F.C., Ehrlich H.P. (1992). Experimental injury procedure included contact third and fourth degree burns at skin hair free back. The burn was applied by copper plates heated in boiling water to 97-100C under ether anesthesia within 20 sec. The size of the lesion was 10-15% of the body.
Results. Hydrogel dressings with an additional absorbent layer had been shown to be effective in positive dynamic regenerative process.
At all stages of the experimental study, absolute fixation of the gauze hydrogel covering on the wounds with no disruption and lysis was observed. The perforated structure of primary dressings and high sorbing ability of polyurethane sponge provided active exudate drain, and made favourable conditions for local therapeutic effect of the regenerative substrate, which is manifested by adjacent tissue edema reduction, normal blood circulation and complete restoration of the affected dermis structure. Thus, a burn wound closure by combination of hydrogel dressing and polyurethane sponge promoted active granulation tissue formation, provided wound healing and anti-inflammatory effects.
Conclusion. The performed research has proved the efficacy of early performance of necrectomy, helped to promote wound healing by means of hydrogel dressings and polyurethane adsorbents.

Keywords: burns, morphometry, necrectomy, hydrogel dressings, polyurethane sponge adsorbents, granulation tissue, anti-inflammatory
p. 109-119 of the original issue
  1. Zonies D, Mack C, Kramer B, Rivara F, Klein M. Verified centers, nonverified centers, or other facilities: a national analysis of burn patient treatment location. J Am Coll Surg. 2010;210(3):299-305.
  2. Lohmeyer JA, Liu F, Krüger S, Lindenmaier W, Siemers F, Machens HG. Use of gene-modified keratinocytes and fibroblasts to enhance regeneration in a full skin defect. Langenbecks Arch Surg. 2011 Apr;396(4):543-50. doi: 10.1007/s00423-011-0761-3.
  3. Shupp JW, Nasabzadeh TJ, Rosenthal DS, Jordan MH, Fidler P, Jeng JC. A review of the local pathophysiologic bases of burn wound progression. J Burn Care Res. 2010 Nov-Dec;31(6):849-73.
  4. Lopes F, Coelho FM, Costa VV, Vieira EL, Silva TA, et al. Hydrogen peroxide resolves neutrophilic inflammation in a model of antigen-induced arthritis in mice. Arthritis Rheum. 2011;10(2):169-72.
  5. Opasanon S, Muangman P, Namviriyachote N. Clinical effectiveness of alginate silver dressing in outpatient management of partial-thickness burns. Int Wound J. 2010 Dec;7(6):467-71. doi: 10.1111/j.1742-481X.2010.00718.x.
  6. Park JB. Healing of extraction socket grafted with deproteinized bovine bone and acellular dermal matrix: histomorphometric evaluation. Implant Dent. 2010 Aug;19(4):307-13. doi: 10.1097/ID.0b013e3181e5abbc.
  7. Povarikhina OA. Sovremennye ranevye pokrytiia v kombustiologii [Modern wound dressings in combustiology] Novye Med Tekhnologii. Novoe Med Oborudovanie. 2007;(3):31-36.
  8. Ryssel H, Germann G, Kloeters O, Gazyakan E, Radu CA. Dermal substitution with Matriderm() in burns on the dorsum of the hand. Burns. 2010 Dec;36(8):1248-53. doi: 10.1016/j.burns.2010.05.003.
  9. Shablin DV, Pavlenko SG, Evglevskii AA, Bondarenko PP, Khuranov AA. Sovremennye ranevye pokrytiia v mestnom lechenii ran razlichnogo geneza [Modern wound dressings in the local treatment of wounds of various genesis]. Fundam Issledovaniia. 201;(12):361-65.
  10. Regas FC, Ehrlich HP. Elucidating the vascular response to burns with a new rat model. J Trauma. 1992 May;32(5):557-63.
  11. Kozinets' GP, Kovalenko ON, Slesarenko SV. Opkova khvoroba [Burn disease]. Mistetstvo Lkuvannia. 2006;(12):9-15.
  12. Lilli R. Patogistologicheskaia tekhnika i prakticheskaia gistokhimiia [Histopathological technique and practical histochemistry]. Moscow, RF: Mir; 1960. 648 p.
  13. Sarkisov DS, Perova IuL. Mikroskopicheskaia tekhnika [Microscopic technique]: ruk dlia vrachei i laborantov. Moscow, RF: Meditsina; 1996. 544 p.
  14. Kornienko VV, Kalshkevich OV, PogorЄlov MV, Oleshko OM. Osoblivost morfogenezu opkovo rani pri zastosuvann khtozanovikh membran v rzn perodi opkovo khvorobi [Features of burn wounds morphogenesis in the application of chitosan membranes at different periods of burn disease]. Morfologia. 2013;7(4):42-50.
Address for correspondence:
46000, Ukraine,
ernopol, ul. Shpitalnaya, d. 2,
Ternopolskiy gosudarstvennyiy meditsinskiy universitet
imeni I.Y. Gorbachevskogo,
kafedra travmatologii i ortopedii s voenno-polevoy khirurgiey,
tel. office: 38 096 7517885,
Kovalchuk Andrey Olegovich
Information about the authors:
Kovalchuk A.O. An associate professor of the traumatology and orthopedic chair with military field surgery of Ternopol State Medical University named after I.Y.Gorbachevsky of the Ministry of Health of Ukraine.
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