Year 2015 Vol. 23 No 5

GENERAL & SPECIAL SURGERY

I.V. SAMSONOVA, V.A. KLOPOVA, I.S. SHEVCHENKO

COLLAGEN IV AND VI EXPRESSION IN PANCREAS AT CHRONIC PANCREATITIS

EE "Vitebsk State Medical University"
The Republic of Belarus

Objectives. To study collagen IV and VI expression in pancreas at chronic pancreatitis (CP).
Methods. Using morphological, immunohistochemical, morphometric and statistical methods the pancreatic tissue specimens of 28 patients with CP, stained with lyophilised monoclonal antibodies Collagen Type IV and Collagen Type VI (Novocastra) have been investigated. Control group included 7 pancreatic head specimens without any pathology of pancreas takon from people died due to by accidents.
Results. Collagen IV-positive reaction was determined as different intensity brown staining of basement membrane in the wall of vessels, ducts and in acini of all pancreatic specimens. At CP the expression level was 750,2±2,86, that corresponded to 1093297,22±3340,36 mkm2 total expression area. In control group the expression was significantly higher – 924,11±8,5, but total expression area was significantly lower – 751045,11±10096,45 mkm2.
In pancreas without pathologic changes expression of collagen VI was mainly determined in the connective tissue cells and was almost absent in the acinar cells. At CP collagen VI positive expression was mainly determined in the basal part of acinar cell and duct epithelium cytoplasm as well as in the connective tissue cells. In control group expression was 615,15 ±19,34 mkm2, at CP – 85524,08± 3433,07 mkm2. The expression total area at CP was 241506 ìêì2, that was 2,8 fold more high comparing with control group.
Conclusion. The chronic pancreatitis is characterized by changes of the Collagen Type IV and Collagen Type VI expression degree, character of distribution and expression area in the pancreas. It is supposed the revealed changes of Collagen Type IV and Collagen Type VI expression to result from collagen synthesis disturbances at chronic inflammation, that can explained by activation of mesenchimal cells, their proliferation and changes of epitheliocytes activity.

Keywords: pancreas, chronic pancreatitis, collagen IV, collagen VI, immunohistochemical investigation, proliferation, epitheliocytes activity
p. 500-505 of the original issue
References
  1. Minushkin ON. Khronicheskii pankreatit: nekotorye aspekty patogeneza, diagnostiki i lecheniia [Chronic pancreatitis: some aspects of the pathogenesis, diagnosis and treatment]. Consilium-Medicum. 2002;(4)1:23-26.
  2. Gubergrits NB, Khristich TN. Klinicheskaia pankreatologiia [Clinical pancreatology]. Donetsk, Ukraina: Lebed; 2000. 416 p.
  3. Layer P, Yamamoto H, Kalthoff L, Clain JE, Bakken LJ, DiMagno EP The different courses of early- and late-onset idiopathic and alcoholic chronic pancreatitis. Gastroenterology. 1994 Nov;107(5):1481-87.
  4. Apte MV, Haber PS, Applegate TL, Norton ID, McCaughan GW, Korsten MA, Pirola RC, Wilson JS. Periacinar stellate shaped cells in rat pancreas: identification, isolation, and culture. Gut. 1998 Jul;43(1):128-33.
  5. Apte M, Pirola R, Wilson J. The fibrosis of chronic pancreatitis: new insights into the role of pancreatic stellate cells. Antioxid Redox Signal. 2011 Nov 15;15(10):2711-22. doi: 10.1089/ars.2011.4079.
  6. Kadono G, Ishihara T, Yamaguchi T, Kato K, Kondo F, Naito I, Sado Y, Saisho H. Immunohistochemical localization of type IV collagen alpha chains in the basement membrane of the pancreatic duct in human normal pancreas and pancreatic diseases. Pancreas. 2004 Jul;29(1):61-66.
  7. Pöschl E, Schlötzer-Schrehardt U, Brachvogel B, Saito K, Ninomiya Y, Mayer U. Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development. Development. 2004 Apr;131(7):1619-28.
  8. Das SK, Varadhan S, Dhanya L, Mukherjee S, Mohana S, Balakrishnan V, Vasudevan DM. Diagnostic efficiency of amylase and type IV collagen in predicting chronic pancreatitis. Indian J Clin Biochem. 2009 Jan;24(1):60-4. doi: 10.1007/s12291-009-0010-5.
  9. Ohlund D, Lundin C, Ardnor B, Oman M, Naredi P, Sund M.Type IV collagen is a tumour stroma-derived biomarker for pancreas cancer. Br J Cancer. 2009 Jul 7;101(1):91-7. doi: 10.1038/sj.bjc.6605107.
  10. Groulx JF, Gagné D, Benoit YD, Martel D, Basora N, Beaulieu JF. Collagen VI is a basement membrane component that regulates epithelial cell-fibronectin interactions. Matrix Biol. 2011 Apr;30(3):195-206. doi: 10.1016/j.matbio.2011.03.002.
  11. öhlund D, Franklin O, Lundberg E, Lundin C, Sund M. Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop. BMC Cancer. 2013 Mar 26;13:154. doi: 10.1186/1471-2407-13-154.
  12. Nishi O, Nishi K, Fujiwara T, Shirasawa E, Ohmoto Y. Effects of the cytokines on the proliferation of and collagen synthesis by human cataract lens epithelial cells. Br J Ophthalmol. 1996 Jan;80(1):63-68.
  13. Rühl M., Sahin E, Johannsen M, Somasundaram R, Manski D, Riecken EO, Schuppan D. Soluble collagen VI drives serum-starved fibroblasts through S phase and prevents apoptosis via down-regulation of Bax. J Biol Chem. 1999 Nov 26;274(48):34361-68.
  14. Gagné D, Groulx JF, Benoit YD, Basora N, Herring E, Vachon PH, Beaulieu JF. Integrin-linked kinase regulates migration and proliferation of human intestinal cells under a fibronectin-dependent mechanism. J Cell Physiol. 2010 Feb;222(2):387-400. doi: 10.1002/jcp.21963.
  15. Sabatelli P, Bonaldo P, Lattanzi G, Braghetta P, Bergamin N, Capanni C, Mattioli E, Columbaro M, Ognibene A, Pepe G, Bertini E, Merlini L, Maraldi NM, Squarzoni S. Collagen VI deficiency affects the organization of fibronectin in the extracellular matrix of cultured fibroblasts. Matrix Biol. 2001 Nov;20(7):475-86.
  16. Schnoor M, Cullen P, Lorkowski J, Stolle K, Robenek H, Troyer D, Rauterberg J, Lorkowski S. Production of type VI collagen by human macrophages: a new dimension in macrophage functional heterogeneity. J Immunol. 2008 Apr 15;180(8):5707-19.
Address for correspondence:
210023, Respublika Belarus',
g. Vitebsk, pr. Frunze, d. 27, UO «Vitebskii
gosudarstvennyi meditsinskii universitet»,
kafedra patologicheskoi anatomii,
e-mail: victoriya_06@tut.by,
Klopova Viktoriia Aleksandrovna
Information about the authors:
Samsonova I.V. PhD, an associate professor, a head of the chair of pathological anatomy EE "Vitebsk State Medical University."
Klopova V.A. PhD, an associate professor of the chair of pathological anatomy EE "Vitebsk State Medical University."
Shevchenko I.S. A senior lecturer of the chair of pathological anatomy EE "Vitebsk State Medical University."
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