Year 2015 Vol. 23 No 5




EE "Vitebsk State Medical University"
The Republic of Belarus

Objectives. To study collagen IV and VI expression in pancreas at chronic pancreatitis (CP).
Methods. Using morphological, immunohistochemical, morphometric and statistical methods the pancreatic tissue specimens of 28 patients with CP, stained with lyophilised monoclonal antibodies Collagen Type IV and Collagen Type VI (Novocastra) have been investigated. Control group included 7 pancreatic head specimens without any pathology of pancreas takon from people died due to by accidents.
Results. Collagen IV-positive reaction was determined as different intensity brown staining of basement membrane in the wall of vessels, ducts and in acini of all pancreatic specimens. At CP the expression level was 750,22,86, that corresponded to 1093297,223340,36 mkm2 total expression area. In control group the expression was significantly higher 924,118,5, but total expression area was significantly lower 751045,1110096,45 mkm2.
In pancreas without pathologic changes expression of collagen VI was mainly determined in the connective tissue cells and was almost absent in the acinar cells. At CP collagen VI positive expression was mainly determined in the basal part of acinar cell and duct epithelium cytoplasm as well as in the connective tissue cells. In control group expression was 615,15 19,34 mkm2, at CP 85524,08 3433,07 mkm2. The expression total area at CP was 241506 2, that was 2,8 fold more high comparing with control group.
Conclusion. The chronic pancreatitis is characterized by changes of the Collagen Type IV and Collagen Type VI expression degree, character of distribution and expression area in the pancreas. It is supposed the revealed changes of Collagen Type IV and Collagen Type VI expression to result from collagen synthesis disturbances at chronic inflammation, that can explained by activation of mesenchimal cells, their proliferation and changes of epitheliocytes activity.

Keywords: pancreas, chronic pancreatitis, collagen IV, collagen VI, immunohistochemical investigation, proliferation, epitheliocytes activity
p. 500-505 of the original issue
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Address for correspondence:
210023, Respublika Belarus',
g. Vitebsk, pr. Frunze, d. 27, UO Vitebskii
gosudarstvennyi meditsinskii universitet,
kafedra patologicheskoi anatomii,
Klopova Viktoriia Aleksandrovna
Information about the authors:
Samsonova I.V. PhD, an associate professor, a head of the chair of pathological anatomy EE "Vitebsk State Medical University."
Klopova V.A. PhD, an associate professor of the chair of pathological anatomy EE "Vitebsk State Medical University."
Shevchenko I.S. A senior lecturer of the chair of pathological anatomy EE "Vitebsk State Medical University."
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