Year 2015 Vol. 23 No 3




EE "Vitebsk State Medical University",
The Republic of Belarus

Objective. To study the CD34 expression in the leg vein wall of patients with the lower limb varicose veins.
Methods. The study included 18 patients who were subjected to the resection of the posterior tibial veins together with phlebectomy because of the diagnosed deep venous valvular insufficiency. For morphological examination the resected specimens of the posterior tibial veins and the distal fragment of the vena saphena magna taken by phlebectomy were used. The samples of the corresponding veins without any pathology obtained from died patients not suffering from the lower limb varicose veins were used for control.
Serial sections were stained with routine histological methods and immunohystochemically using monoclonal antibodies against CD34. The degree of CD34 expression in the specimens was evaluated by light microscopy.
Results. In the posterior tibial vein wall of the patients with lower limb varicosity and deep venous valvular insufficiency, the degree of its expression in the endothelium comparing was different from the control group. The total area of expression was 0,00850,0004 μm2, in control samples it was significantly less 0,0040,0003 μm2; (p<0,05). In the vena saphena magna the specimens taken from the patients with varicose veins, CD34 expression area was also significantly higher than in the control group 0,01160,0009 μm2 (in control group 0,0050,0004 μm2; p<0,05). Comparison of CD34 expression in the specimens of the vena saphena magna and posterior tibial veins taken from the patients with lower limb varicose veins showed reliably higher parameters in the vena saphena magna (p<0,05).
Conclusion. At lower limbs superficial varicose veins the unidirectional changes in the endothelium of the superficial and deep leg veins are developing; its proved by change in the CD34 expression on the endothelial cell surface.

Keywords: primary varicose veins, endothelium, vein wall, immunohistochemistry, CD34 expression, light microscopy, phlebectomy
p. 302-308 of the original issue
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Address for correspondence:
210023, Respublika Belarus,
g. Vitebsk, pr. Frunze, d. 27,
UO "Vitebskiy gosudarstvennyiy meditsinskiy universitet",
kafedra obschey khirurgii,
Sushkov Sergey Albertovich
Information about the authors:
Sushkov S.A.PhD, an associate professor, Vice-rector (Science) of EE "Vitebsk State Medical University".
Samsonova I.V. PhD, an associate professor, a head of the pathological anatomy chair of EE "Vitebsk State Medical University".
Galishevich M.M. The 6th-year student of the medical faculty of EE "Vitebsk State Medical University".
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