Year 2015 Vol. 23 No 3

SCIENTIFIC PUBLICATIONS
EXPERIMENTAL SURGERY

A.V. BURAVSKY 1, E.V. BARANOV 1, S.I. TRETYAK 1, Z.B. KVACHEVA 2

INFLUENCE OF MONOCHROMATIC POLARISED LIGHTEMITTING DIODE RADIATION ON THE PROLIFERATION OF CULTIVATED HUMAN DERMAL FIBROBLASTS

EE "Belarusian State Medical University" 1,
SSE "Institute of Biophysics nd Cell Engineering
of NAS of Belarus" 2,
The Republic of Belarus

Objectives. To determine the physical parameters of a polarized light-emitting diode radiation (PLEDR) providing the stimulation of the proliferative activity (PA) of the human dermal fibroblast proliferation (HSF) in the cell culture.
Methods. Cultivated human dermal fibroblasts had not been radiated in the control group. Monolayer cell cultures in 16 experimental groups were radiated with monochromatic PLEDR, separately or sequentially using wavelengths λ1=(0,6300,03) m λ2=(0,4050,015) m. The study of the mitotic and proliferative activity was carried out in 24-48 hours after the exposure; the morphology of the cells had been evaluated intravitally under a phase-contrast microscopy.
Results. The most pronounced stimulation of mitotic actirity (MA) was observed in the experimental groups after PLEDR with the wavelength of λ1=(0,6300,03) m at radiant energy density (RED) 10 J/cm2 and 15 J/cm2: the mitotic index 24 hours after radiation was 29,73,10 and 63,04,44, respectively, being reliably higher than in control. In 48 hours after radiation the evaluation of morphological changes and accumulation of cells in these groups had been revealed the "saturation cell density" increase in the monolayer due to their number growth at 1.6 folds in RED 10 J/cm2 and at 2.0 folds in RED 15 J/cm2 justifying increasing of proliferative activity (PA) cultured fibroblasts under the indicated photo-radiation parameters. In other experimental groups statistically significant reduction of MA or lack of difference towards the control group were observed.
Conclusion. The conducted research has confirmed the monochromatic PLEDR possibility to stimulate HSF proliferation in culture under the specific exposure parameters.

Keywords: human skin fibroblasts, monolayer cell culture, the morphology of the cell cultures, phase contrast microscopy, mitotic activity, polarized light-emitting diode radiation, proliferative activity
p. 247-255 of the original issue
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Address for correspondence:
220116, Respublika Belarus,
g. Minsk, pr. Dzerzhinskogo, d. 83,
UO "Belorusskiy gosudarstvennyiy meditsinskiy universitet",
2-ya kafedra khirurgicheskih bolezney,
tel. mob. 375 29 622-57-81,
e-mail: buravskyav@mail.ru,
Buravsky Aleksandr Vladimirovich
Information about the authors:
Buravsky A.V. An assistant of the 2nd chair of surgical diseases of EE "Belarusian State Medical University".
Baranov E.V. PhD, an associate professor of the 2nd chair of surgical diseases of EE "Belarusian State Medical University".
Tretyak S.I. Corresponding member of NAS of Belarus, MD, professor, a head of the 2nd chair of surgical diseases of EE "Belarusian State Medical University".
Kvacheva Z.B. PhD, a leading researcher of the laboratory of cell molecular biology of SSE "Institute of Biophysics and Cell Engineering of NAS of Belarus".
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