Year 2014 Vol. 22 No 1




Center of New Medical Technologies of the Institute of Chemical Biology and Fundamental Medicine of SB RAS, Novosibirsk,
The Russian Federation

Objectives. To estimate the reaction of rats tissues to the lactic acid polymers (PLA) implant and peculiarities of the processes of its resorption morphologically.
Methods. The experiments were carried out on 24 male rats inbred line of WAG weighing 180-200 g at the age of 6 months. Using the inhalation ester anesthesia the PLA fragment was implanted into the subcutaneous adipose tissue over the right shoulder blade. The animals were discharged from the experiment on the 1st, 2nd, 6th and 12th months after the operation. 6 rats were used for every point of the research. The implanted PLA together with the surrounding tissues were taken for the investigation, which were studied microscopically.
Results. It was revealed that after the PLA implantation it is encapsulated by the connective tissue. If the PLA fragment has the keen edges, a thick capsule is formed with the expressed inflammatory infiltration and the sclerosis of surrounding tissues. If the implant was without keen edges a thin capsule is formed and the inflammatory level is minimal. In all cases the giant cells of foreign bodies are in the capsule and surround it. Formation of the adhered multinuclear macrophages in the tissues surrounding the capsule is definitely due to the presence of small polymer fragments there. Gradually within 6 months the activity of inflammatory process reduced so as the thickness of the delimited capsule and strength of its attaching to the surrounding tissues. Expressiveness of the inflammation sharply increased up to 12 months after implantation, when as the result of the phagocytes enzymes action and deformation by the capsule PLA is either fragmented or diluted. Probably, a sharp increase of the inflammation reaction level by the time of the observation period termination correlated with the polymer degradation level.
Conclusions. Complete PLA destruction and absorption in an organism has not occurred, such polymers aren't fully biodegraded and destructed for a long time and generally via macrophage lysis. PLA use has to be critically reconsidered.

Keywords: polylactide, degradation, encapsulation, implantation, granulomatous inflammation
p. 26 32 of the original issue
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Address for correspondence:
630090, Rossiiskaia Federatsiia, g. Novosibirsk, pr. akad. Lavrent'eva, d. 8, Institut khimicheskoi biologii i fundamental'noi meditsiny SO RAN, Tsentr novykh meditsinskikh tekhnologii,
Mayborodin Igor Valentinovich
Information about the authors:
Maiborodin I.V. MD, professor, a leading researcher of the stem cell laboratory of ICBFM SB RAS.
Kuznetsova I.V. PhD, a researcher of the stem cell laboratory of ICBFM SB RAS.
Beregovoy E.A. PhD, an applicant for Doctors degree of the regenerative surgery laboratory of ICBFM SB RAS.
Shevela A.I. MD, professor, Honored Physician of RF, Deputy Director of ICBFM SB RAS (Science).
Maiborodina V.I. MD, a senior researcher of the stem cell laboratory of ICBFM SB RAS.
Manaev A.A. A post-graduate student of the stem cell laboratory of ICBFM SB RAS.
Barannick M.I. PhD, an applicant for Doctors degree of the stem cell laboratory of ICBFM SB RAS.
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