Year 2013 Vol. 21 No 5

EXPERIMENTAL SURGERY

S.L. ZYBLEV, Z.A. DUNDAROV

THE STATE OF METABOLISM IN EXPERIMENTAL ACUTE MASSIVE BLOOD LOSS DEPENDING ON THE CONDUCTED THERAPY

EE "Gomel State Medical University",
The Republic of Belarus

Objectives. To study the impact of the conducted therapy on the metabolic state of animals with acute massive blood loss in experiment.
Methods. The experimental group of animals with acute blood loss was divided into five subgroups. The first subgroup was composed of the rats received no any treatment. The second subgroup included the rats underwent to the physiological saline injections after bleeding procedure. The third subgroup consisted of the rats underwent the injections of autologous blood with physiological saline. The fourth subgroup was made up of the rats with reinfusion of the whole autologous blood. The fifth subgroup consisted of the rats to whom the antioxidant complex Cytoflavin has been injected after bleeding procedure. After the treatment red blood cell count and concentration of hemoglobin in the blood as well as indices of acid-base balance, biomedical parameters and antioxidant activity (AOA) of blood serum have been evaluated. The received data were compared with those of healthy animals (control group).
Results. It was experimentally proved that the injections of saline solution and reinfusion of autologous blood at the rate of 50% out of the blood loss volume with an equal volume of physiological saline did not significantly affect on the metabolic processes in animals after acute massive blood loss. Free-radical processes in the body with the prevalence of peroxidation and the formation of oxidative stress couldnt be adjusted even by massive whole autologous blood transfusion. The received data indicate the high efficiency of the antioxidant complex Cytoflavin for the correction of metabolic post hemorrhagic disorders. The introduction of the drug Cytoflavin eliminates the prevalence of peroxidation processes and stops oxidative stress.
Conclusions. The activation of peroxidation leads to the growth of uric acid concentration as an active natural antioxidant. The injection of saline solutions in massive blood loss is unable to compensate the acid-base balance of the body, supply of antioxidant potential and reduce peroxidation processes. Massive blood transfusion equal to the volume of acute blood loss does not lead to a stabilization of free-radical processes in the body. The use of antioxidants in acute massive blood loss is an effective measure to prevent and relieve oxidative stress.

Keywords: blood loss, hemorrhagic shock, oxidative stress, acid-base balance, blood transfusion, cytoflavin
p. 3 10 of the original issue
References
  1. Iakovlev AIu, Emel'ianov NV, Mukhina IV, Dvornikov AV, Snopova LB, Kalent'ev GV, Riabikov DV, Mineeva NV. Vybor infuzionnogo preparata dlia profilaktiki poliorgannoi nedostatochnosti pri ostroi massivnoi krovopotere (eksperimental'noe issledovanie) [Choosing an infusion drug for the prevention of polyorgan failure in acute massive blood loss (experimental study)]. Obshch Reanimatol. 2010;6(3):4851.
  2. Kligunenko EN, Kravets OV. Intensivnaia terapiia krovopoteri [Intensive therapy of blood loss]. Moscow, RF: MEDpress-inform; 2005. 112 p.
  3. Butrov AV, Borisov AIu, Galenko SV. Ratsional'naia infuzionnaia terapiia u bol'nykh v kriticheskikh sostoianiiakh [Rational influsion therapy in critically ill patients.]. Trudn Patsient. 2006;4(10):1923.
  4. Golubev AM, Tamaeva FA. Korrektsiia metabolicheskikh narushenii v pochkakh pri ostroi massivnoi krovopotere (eksperimental'noe issledovanie) [Correction of metabolic abnormalities in the kidneys in acute massive blood loss (experimental study)]. Obshch Reanimatol. 2007;3(5-6):3842.
  5. Moroz VV. Strategiia i taktika primeneniia antigipoksantov pri kriticheskikh sostoianiiakh. V kn.: Fundamental'nye problemy reanimatologii (Izbrannye lektsii i obzory) [Strategy and tactics antihypoxants in critical conditions. In.: Fundamental Problems of Critical Care Medicine (Selected lectures and reviews)]. Tr Instituta obshchei reanimatologii RAMN. Moscow, RF: 2005;(4). p.21020.
  6. Maevskii EI, Grishina EV, Rozenfel'd AS, Ziakun AM, Vereshchagina IM, Kondrashova MN. Anaerobnoe obrazovanie suktsinata i oblegchenie ego okisleniia vozmozhnye mekhanizmy adaptatsii kletki k kislorodnomu golodaniiu [Anaerobic formation of succinate and facilitate its oxidation. Possible adaptation mechanisms of cell to hypoxia]. Biofizika 2000 Dek;1(3):3236.
  7. Luk'ianova LD, Rol' bioenergiticheskikh narushenii v patogeneze gipoksii [The role of bioenergetic disorders in the pathogenesis of hypoxia]. Patol Fiziol i Eksperiment Terapiia. 2004;(2):211.
  8. Morgunov SS. Korrektsiia tkanevoi gipoksii i protsessov svobodnoradikal'nogo okisleniia pri gastroduodenal'nykh krovotecheniiakh [Correction of tissue hypoxia and free radical oxidation processes in the gastroduodenal hemorrhage]. Khirurgiia. Zhurn im NI Pirogova.2011;(9):7175.
  9. Silina EV, Stupin VA, Sabirov MA, Bolevich SB, Smirnova GO, Siluianov SV, Martirosov AV, Men'shova NI. Svobodnoradikal"nye protsessy u bol'nykh s zheludochno-kishechnymi krovotecheniiami [Free radical processes in patients with gastrointestinal bleeding]. Khirurgiia. Zhurnal im NI Pirogova.2011;(12): 6470.
  10. Stupin VA, Siluianov SV, Afanas'ev VV, Baglaenko MV, Sabirov MA, Smirnova GO. Osobennosti konservativnoi terapii patsientov s krovotochashchimi iazvami zheludka i dvenadtsatiperstnoi kishki [Features of the conservative treatment of patients with bleeding ulcers of the stomach and duodenum]. Farmateka. 2011;(2):5863.
  11. Zyblev SL, Dundarov ZA. Antioksidantnaia aktivnost' krovi bol'nykh s ostrym gastroduodenal'nym krovotecheniem [The antioxidant activity of the blood of patients with acute gastroduodenal bleeding]. Khirurgiia. Vostochn Evropa. 2013;(1):1223.
  12. Sirota TV. Sposob opredeleniia antioksidantnoi aktivnosti superoksiddismutazy i khimicheskikh soedinenii [Method of determination of superoxide dismutase antioxidant activity and chemical compounds]: pat. 2144674 RF, MPK7 G01N33/52, G01N33/68/ 99103192/14; zaiavl. 24.02.1999; opubl. 20.01.2000. 2000(2)2: 266 p.
  13. Gritsuk AI, Cupoma TB, Dravitsa LV, Kreddok EA. Otsenka sostoianiia antioksidantnoi aktivnosti sleznoi zhidkosti [Assessment of the antioxidant activity of tear fluid]. Biomed Khim. 2006;(6)52:60107.
Address for correspondence:
246000, Respublika Belarus', g. Gomel', ul. Lange, d. 5, UO Gomel'skii gosudarstvennyi meditsinskii universitet, kafedra khirurgicheskikh boleznei 2 s kursom detskoi khirurgii,
e-mail: S.zyblev@yandex.ru,
Zyblev Sergei Leonidovich
Information about the authors:
Zyblev S.L. An assistant of the chair of surgical diseases 2 with the course of pediatric surgery of EE "Gomel State Medical University".
Dundarov Z.A. MD, professor, a head of the chair of surgical diseases 2 with the course of pediatric surgery of EE "Gomel State Medical University". Dundarov Z.A. MD, professor, a head of the chair of surgical diseases 2 with the course of pediatric surgery EE "Gomel State Medical University".
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