Year 2013 Vol. 21 No 4




EE Vitebsk State Medical University
The Republic of Belarus

Objectives. To access the efficacy of platelet-rich plasma (PRP) application in treatment of knee hyaline cartilage defects in a rabbit model.
Methods. Chinchilla rabbits (n=10) were subdivided into two groups. The full-thickness cartilage defect of the load-bearing surface of the patello-femoral joint of left femur was created in the first group of animals (n=5). Partial-thickness cartilage defect was created at the same area in the second group of animals (n=5). 0.5 mL of PRP was injected in a joint cavity after wound closure. In order to form a control group the same cartilage defect was created at the contralateral knee joint and intra-articular injections of 0.5 ml of normal saline (0.9%) were injected. In 11 weeks after the operation the macroscopic and microscopic examination of cartilage defect area has been performed with the evaluation of characteristics of repair tissue by the ODriscoll scale.
Results. Group of animals underwent the treatment of full-thickness cartilage defect with PRP characterized by significantly higher values of categories Morphology of tissue, Matrix staining, Integrity of structure, Thickness of neo-formed cartilage, Clustering of chondrocytes, Inflammation (p<0.05) and final value of the ODriscoll Score (p<0.05) in comparison with the control group. In the group of animals with partial-thickness cartilage defect treated with PRP the values were significantly higher than in the categories Inflammation and Hypocellularity (p<0.05) and final value of the ODriscoll Score (p<0.05) compared with the control one. There were no any complications associated with intra-articular PRP injections.
Conclusions. Intra-articular PRP injections improve cartilage regeneration in both full-thickness and partial-thickness cartilage defects of the knee. It is a safe and effective method which can be used for the treatment of knee cartilage injuries.

Keywords: knee joint, cartilage defect, platelet-rich plasma
p. 3 9 of the original issue
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Address for correspondence:
210023, Respublika Belarus', g. Vitebsk, pr. Frunze, d. 27, UO Vitebskii gosudarstvennyi meditsinskii universitet, kafedra travmatologii, ortopedii i VPKh,
Mastykov Anton Nikolaevich
Information about the authors:
Mastykau A.N. A post-graduate student of the traumatology, orthopedics and military-field medicine of EE Vitebsk State Medical University.
Deykalo V.P. MD, professor, Rector of EE Vitebsk State Medical University.
Samsonova I.V. PhD, associate professor, a head of the pathologic anatomy chair of EE Vitebsk State Medical University.
Balaboshka K.B. PhD, associate professor of the traumatology, orthopedics and military-field medicine of EE Vitebsk State Medical University.
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