Year 2013 Vol. 21 No 1

EXPERIMENTAL SURGERY

A.A. GLUKHOV, N.T. ALEKSEEVA, A.P. OSTROUSHKO

MORPHOFUNCTIONAL CHANGES IN TISSUES AT WOUNDS HEALING ON THE BACKGROUND OF THE PLATELET CONCENTRATE USE

SBEE HPE Voronezh State Medical Academy named after N.N. Burdenko
The Russian Federation

Objectives. To assess the tissue morphofunctional changes during the healing of wounds on the background of the selective and the combined platelet concentrate (PC) and hydroimpulsive sanation (HIS) usage depending on the type of a wound and a phase of a wound process.
Methods. The studies were conducted on 196 adult male rats weighing 250-280 g, who were applied a standard linear wound (1,0×0,5 cm) on the anterior side of the thigh under anesthesia. The first unit consisted of one control and two experimental groups in which reparative processes in aseptic wounds on the background of HIS and C were evaluated.
In the first experimental group the PC with a concentration of at least 1 million/ml was used for treatment. In the second group HIS of a wound was applied. The second unit consisted of one control and 3 experimental groups and was devoted to studies of impact of PC and HIS on the reparative processes in the purulent wounds.
After purulent wounds modeling in the 1st experimental group the PC was applied to the wound; in the 2nd group HIS of a wound was applied. In the 3rd group firstly HIS was conducted and then PC was applied. In the control groups no treatment was carried out. Planimetric, histological, immunohistochemical methods of investigation were used.
Results. The conducted morphological study permitted to determine that the aseptic wounds treatment on the background PC accelerates regeneration, increasing the metabolic processes in the sprout layer of the epidermis, stimulates angiogenesis within the dermis. Healing of purulent wounds appears to be faster in case of the combined HIS and PC usage. These morphological studies data correlate with the results of the dynamics of change in the area of wounds from that of marker protein-synthetic processes.
Conclusions. Selective PC usage potentiates regenerative processes in aseptic wounds, the combined use of HIS and PC is considered to be more effective in septic wounds.

Keywords: healing of wounds, hydroimpulsive sanation, platelet concentrate
p. 15 22 of the original issue
References
  1. Bulynin VI, Bulynin VI, Glukhov AA, Moshurov IP. Lechenie ran [The treatment of wounds]. Voronezh, RF: Voronezh gos un-t; 1998. 248 p.
  2. Zemskov MA, Khoroshilov AA, Il'ina EM, Domnich OA. Gnoino-vospalitel'nye zabolevaniia aktual'nye problemy khirurgii.[Purulent and inflammatory diseases - the current problems of surgery]. Vestn Eksperim i Klin khirurgii. 2011;24(3):46872.
  3. Rany i ranevaia infektsiia: ruk dlia vrachei [Wounds and wound infection: a guide for physicians]. Kuzin MI, Kostiuchenok BM, red. 2-e izd, pererab i dop. Moscow, RF: Meditsina; 1990. 592 p .
  4. Vinnik IuS, Salmina AB, Drobushevskaia AI, Tepliakova OV, Pozhilenkova EA, Zykova LD. Kletochnye tekhnologii i tkanevaia inzheneriia v lechenii dlitel'no nezazhivaiushchikh ran [Cell technology and tissue engineering in the treatment of nonhealing wounds ]. Vestn Eksperim i Klin Khirurgii. 2011;IV(2):39297.
  5. Gostishchev VK. Operativnaia gnoinaia khirurgiia: ruk dlia vrachei [Operative purulent surgery: a guide for physicians]. Moscow, RF: Meditsina; 1996. 416 p.
  6. Zatolokin VD, Moshkin AS. Vliianie vodnykh dispersnykh oksidnykh nanostruktur metallov na lechenie gnoinykh ran [Effect of water -dispersed metal oxide nanostructures for treatment of septic wounds]. Vestn Eksperim i Klin Khirurgii. 2010;3(1):4451.
  7. Larichev AB. Shishlo VK, Lisovskii AV, Chistiakov AL, Vasil'ev AA. Profilaktika ranevoi infektsii i morfologicheskie aspekty zazhivleniia asepticheskoi rany [Prevention of wound infection and morphological aspects of aseptic wound healing]. Vestn Eksperim i Klin Khirurgii. 2011;IV(4):72833.
  8. Lutsevich OE, Tamrazova OB, Shikunova AIu, Pleshkov AS, Ismailov GI O, Vorotilov IuV, Tolstykh PI. Sovremennye vzgliady na patogenez i lechenie gnoinykh ran [Current views on the pathogenesis and treatment of purulent wounds]. Khirurgiia Zhurn im NI Pirogova. 2011;(5):7277.
  9. Lacci KM, Dardik A. Platelet-rich plasma: support for its use in wound healing. Yale J Biol Med. 2010 Mar;83(1):19.
  10. Han T, Wang H, Zhang YQ. Combining platelet-rich plasma and tissue-engineered skin in the treatment of large skin wound. J Craniofac Surg. 2012 Mar;23(2):43947.
  11. Serov VV, Shekhter AB. Soedinitel'naia tkan' (funktsional'naia morfologiia i obshchaia patologiia) [Connective tissue (the functional morphology and general pathology)]. Moscow, RF: Meditsina; 1981. 312 p.
  12. Maianskii DN. Khronicheskoe vospalenie [Chronic inflammation]. Moscow, RF: Meditsina, 1991. 272 p.
  13. Bozo IIa, Deev RV, Pinaev GP. Fibroblast spetsializirovannaia kletka ili funktsional'noe sostoianie kletok mezenkhimal'nogo proiskhozhdeniia [Fibroblast - a specialized cell or functional state of cells of mesenchymal origin]. Tsitologiia. 2010;52(2):99109.
  14. Omel'ianenko NP, Slutskii LI. Soedinitel'naia tkan' (gistofiziologiia i biokhimiia): v 2 t [Connective tissue (histophysiology and biochemistry) in 2 vol]. Mironov SP, red. Moscow, RF: Izvestiia; 2009;1. 379 p.
Address for correspondence:
394000, Rossiiskaia Federatsiia, g.Voronezh, ul. Studencheskaia, d.10, GBOU VPO Voronezhskaia gosudarstvennaia meditsinskaia akademiia, kafedra obshchei khirurgii,
e-mail: alexeevant@list.ru
Glukhov Alexandr Anatol'evich
Information about the authors:
Glukhov A.A. MD, professor, a head of the general surgery chair of SBEE HPE Voronezh State Medical academy named after N.N. Burdenko
Alekseeva N.T. PhD, associate professor of the normal human anatomy chair of SBEE HPE Voronezh State Medical academy named after N.N. Burdenko
Ostroushko A.P. PhD, assistant of the general surgery chair of SBEE HPE Voronezh State Medical Academy named after N.N. Burdenko
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