Novosti
Khirurgii

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Year 2018 Vol. 26 No 2

GENERAL & SPECIAL SURGERY

V.M. CHUMAKOV, O.L. SYTNIK, V.I. BUGAIOV

POLYMORPHISM OF IL-8 CYTOKINE GENE IN PATIENTS WITH PANCREATOGENIC PERITONITIS

Sumy State University, Medical Institute,
Ukraine

Objective. This preliminary study was conducted to assess the possible association of IL-8 (-251À/Ò) polymorphism with clinical course and outcome of acute pancreatitis aggravated by pancreatogenic peritonitis.
Methods. Data for the study were DNA samples, received from the leucocytes of 143 humans: 83 patients with acute pancreatitis aggravated by pancreatogenic peritonitis, 60 healthy blood donors without acute pancreatitis in the anamnesis served as controls. IL-8 (-251À/Ò) polymorphism detection was made with polymerase chain reaction with further length analysis of the restriction fragments.
Results. The analysis of frequency of allelic variants of the cytokine gene IL-8 (-251À/Ò) revealed that genotype A/T was the dominant variant (45%) among healthy blood donors. Distribution of IL-8 (-251À/Ò) polymorphism among the patients with pancreatogenic peritonitis without and after surgical treatment is characterized by dominance of genotype T/T in group after surgical treatment (ð<0.05). This may indicate association of genotype T/T and unfavorable clinical course of pancreatogenic peritonitis (OR>1). Among patients after surgical treatment genotype A/T was less often met in comparison with the group without it (p<0.05). This may indicate the association of genotype A/T and favorable clinical course of pancreatogenic peritonitis (OR<1). Genotype A/A was rarely registered, which may be due to regional peculiarities of the patient’s genotype.
Conclusions. This preliminary study suggests that the identification of genetic polymorphism of IL-8 (-251A/T)
may be informative and serve as an additional criterion to predict both the clinical course and outcome of pancreatogenic peritonitis; it may also specify indications for surgical treatment. However, the possible role of IL-8 (-251A/T) cytokine polymorphism in the outcome of pancreatogenic peritonitis requires further carefully planned cohort investigations.

Keywords: acute pancreatitis, pancreatogenic peritonitis, interleukin-8, gene polymorphism, immune response, cytokines
p. 163-168 of the original issue
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Address for correspondence:
40007, Ukraine,
Sumy, Rimsky-Korsakov Str., 2,
Sumy State University,
Department of Surgery and Oncology,
Tel. office: +380 542 64-03-18,
e-mail: triftonte@gmail.com,
Chumakov Volodymyr M.
Information about the authors:
Chumakov Volodymyr N., Assistant of the Department of Surgery and Oncology, Sumy State University, Sumy, Ukraine.
https://orcid.org/0000-0002-3022-7148
Sytnik Oleksandr L., PhD, Associate Professor of the Department of Surgery and Oncology, Sumy State University, Sumy, Ukraine.
https://orcid.org/0000-0001-6855-2467
Bugaiov Volodymyr I., PhD, Associate Professor of the Department of Surgery and Oncology, Sumy State University, Sumy, Ukraine.
https://orcid.org/0000-0002-5681-3153
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