Novosti
Khirurgii
This journal is
indexed in Scopus









Year 2014 Vol. 22 No 2

EXPERIMENTAL SURGERY

DOI: http://dx.doi.org/10.18484/2305-0047.2014.2.150   |  

R.E. KALININ, I.A. SUCHKOV, A.N. NOVIKOV, A.S. PSHENNIKOV

MODELING AND CORRECTION OF EXPERIMENTAL VENOUS ENDOTHELIAL DYSFUNCTION

HBEE HPE Ryazan State Medical University named after academician I.P. Pavlov,
The Russian Federation

Objectives. To estimate the different ways of the venous endothelial dysfunction modeling in experiment and study its further correction using the micronized purified flavonoid fraction (MPFF).
Methods. The experiment was carried out on 105 Wistar rats divided into 3 groups: the 1st one the model of deep venous thrombosis, the 2nd the model of the postthrombotic syndrome and the 3rd group the model of L-NAME-induced endothelial dysfunction. The micronized purified flavonoid fraction was administrated within 6 months in the dosage 100 mg/kg/day. At the scheduled time the blood sampling was performed in 7 animals with their further euthanasia. The following metabolites have been determined: super oxide dismutase (SOD), malone dialdehyde (MDA) and nitric oxide (II) (NO).
Results. In all groups a reliable reduction of metabolites of nitric oxide (II), increasing of MDA level and, as a consequence, compensatory activation of antioxidant system, manifested in the increase of SOD level have been revealed. The authentic decreasing of NO and SOD levels, and increasing of MDA level and as the result a compensatory activity of antioxidant system, manifesting in the increase in the SOD level have been found out in all groups. The administration of the micronized purified fraction of flavonoids has led to the reliable (<0,05) increasing of NO synthesis and decreasing of MDA and SOD levels in all groups during the experiment. All studied readings had reached their initial levels by the 1st month of the research and remained on these levels within whole period of the experiment. It is likely, that SOD activity on the background of the micronized purified fraction of flavonoids administration decreased due to lipid peroxidation products reduction being a prerequisite for positive effects of NO realization.
Conclusions. The performed research has proved that the reproduced models allow making all requisite conditions for studying different diseases of the venous system in experiment. The administration of the micronized purified fraction of flavonoids makes a positive influence on the endothelium functional state characterizing by the increase of nitric oxide (II) level and reduction of free-radical oxidation.

Keywords: endothelial dysfunction, micronized purified fraction of flavonoids, thrombosis, postthrombotic syndrome
p. 150 154 of the original issue
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Address for correspondence:
390026, Rossiiskaia Federatsiia, g. Riazan', ul. Vysokovol'tnaia, d. 9, kafedra angiologii, sosudistoi, operativnoi khirurgii i topograficheskoi anatomii GBOU VPO Riazanskii gosudarstvennyi meditsinskii universitet im. akad. I.P. Pavlova,
e-mail: anovikovn@rambler.ru,
Novikov Aleksei Nikolaevich
Information about the authors:
Kalinin R.E. MD, professor, a head of the angiology, vascular, operative surgery and topographic anatomy chair of SBEE HPE Ryazan State Medical University named after academician I.P. Pavlov.
Suchkov I.A. PhD, an associate professor of the angiology, vascular, operative surgery and topographic anatomy chair of SBEE HPE Ryazan State Medical University named after academician I.P. Pavlov.
Novikov A.N. An intramural post-graduate student of the angiology, vascular, operative surgery and topographic anatomy chair of SBEE HPE Ryazan State Medical University named after academician I.P. Pavlov.
Pshennikov A.S. PhD, an assistant of the angiology, vascular, operative surgery and topographic anatomy chair of SBEE HPE Ryazan State Medical University named after academician I.P. Pavlov.
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