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Khirurgii

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Year 2013 Vol. 21 No 2

TRANSPLANTOLOGY

S.V. SPIRYDONAU1, O.A. YUDINA2, A.P. SHKET1 , Y. M. CHESNOV1 , S.I. DRYK3 , A. V. ADZINTSOU1 , N.N. SHCHATSINKA1 , N.N. ZUE

VARIANTS OF CRYOPRESERVED ALLOGRAFTS PREPARATION BEFORE IMPLANTATION

Objective. To optimize a defrosting regimen of cryopreserved allografts.
Material and methods. 27 cryopreserved allografts of the aortic and pulmonary valves were included in the study. Allograft sterilization was performed in antibiotic solution, containing nutritive media, cefazolin, metronidazole and fluconazole. Cryoconservation was done using program freezer at cooling rate of 1°C per minute until -80°Ñ. Dimethylsulfoxide was used as a cryoprotectant. Three different thawing conditions were applied: 1) at 36-42°Ñ (n=10) within 15 minutes, 2) at 18-20°Ñ (n=7) within 35 minutes, 3) at 8-10°Ñ (n=10) within one hour (modification of RSPC «Ñardiology»). The removal of cryoprotectant was performed with gradual reduction of its concentration.
Results. The diameter and shape of allografts remained the same after completion of defrosting process in comparison with the primary patterns. Defrosted allografts had identically dense consistency at using various defrosting variants. The performed macroscopic evaluation permits to confirm that defrosting at 8-10°Ñ doesn’t lead to allîgraft wall ruptures.
The strength characteristics investigation during hydraulic tests revealed the absence of any differences in allografts durability after thawing in different temperature conditions. Histological evaluation of the allografts defrosted at 8-10°Ñ and at 18-20°Ñ didn’t reveal any structure changes in comparison with native valves.
Conclusions. Defrosting of cryopreserved aortic and pulmonary allografts at the temperature from 8°Ñ to 10°Ñ within 60 minutes turns up to be optimal due to providing allograft integrity, endurance and its histological structure integrity.

Keywords: cryopreserved allografts, preparation of preimplantation
p. 76 – 81 of the original issue
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Address for correspondence:
220036, Respublika Belarus', g. Minsk, ul. R. Liuksemburg, d. 110, GU RNPTs «Kardiologiia», 2-e kardiokhirurgicheskoe otdelenie,
e-mail: spiridonov@telegraf.by,
Spiridonov Sergei Viktorovich
Information about the authors:
Spirydonau S.V. PhD, cardiosurgeon of RSPC "Cardiology".
Yudina O.A. PhD, a head of the general pathology department of ME “City Pathological-anatomical bureau”.
Shket A.P. PhD, cardiologist, head of the 2nd cardiac surgery department of RSPC "Cardiology".
Chesnov Y.M. MD, cardiosurgeon of RSPC "Cardiology".
Dryk S.I. A head of the department of separation and cryoconservation of the bone marrow of ME “The 9th city clinical hospital”.
Adzintsou A. V. Cardiologist of RSPC "Cardiology".
Shchatsinka N.N. Cardiologist of RSPC "Cardiology".
Zuenok N.N. Physician of the department of separation and cryoconservation of the bone marrow of ME “The 9th city clinical hospital”.
Ostrovsky Y.P. Corresponding member of NAS, MD, professor, a head of the laboratory of cardiac surgery of SE RSPC "Cardiology", head of the cardiac surgery chair of SEE “Belarusian Medical Academy of Post-graduate Education”.
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