Novosti
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This journal is indexed in Scopus |
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Year 2013 Vol. 21 No 1
EXPERIMENTAL SURGERY
A.A. GLUKHOV, N.T. ALEKSEEVA, A.P. OSTROUSHKO
MORPHOFUNCTIONAL CHANGES IN TISSUES AT WOUNDS HEALING ON THE BACKGROUND OF THE PLATELET CONCENTRATE USE
SBEE HPE “Voronezh State Medical Academy named after N.N. Burdenko”
The Russian Federation
Objectives. To assess the tissue morphofunctional changes during the healing of wounds on the background of the selective and the combined platelet concentrate (PC) and hydroimpulsive sanation (HIS) usage depending on the type of a wound and a phase of a wound process.
Methods. The studies were conducted on 196 adult male rats weighing 250-280 g, who were applied a standard linear wound (1,0×0,5 cm) on the anterior side of the thigh under anesthesia. The first unit consisted of one control and two experimental groups in which reparative processes in aseptic wounds on the background of HIS and ÐC were evaluated.
In the first experimental group the PC with a concentration of at least 1 million/ml was used for treatment. In the second group HIS of a wound was applied. The second unit consisted of one control and 3 experimental groups and was devoted to studies of impact of PC and HIS on the reparative processes in the purulent wounds.
After purulent wounds modeling in the 1st experimental group the PC was applied to the wound; in the 2nd group HIS of a wound was applied. In the 3rd group firstly HIS was conducted and then PC was applied. In the control groups no treatment was carried out. Planimetric, histological, immunohistochemical methods of investigation were used.
Results. The conducted morphological study permitted to determine that the aseptic wounds treatment on the background PC accelerates regeneration, increasing the metabolic processes in the sprout layer of the epidermis, stimulates angiogenesis within the dermis. Healing of purulent wounds appears to be faster in case of the combined HIS and PC usage. These morphological studies data correlate with the results of the dynamics of change in the area of wounds from that of marker protein-synthetic processes.
Conclusions. Selective PC usage potentiates regenerative processes in aseptic wounds, the combined use of HIS and PC is considered to be more effective in septic wounds.
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394000, Rossiiskaia Federatsiia, g.Voronezh, ul. Studencheskaia, d.10, GBOU VPO «Voronezhskaia gosudarstvennaia meditsinskaia akademiia», kafedra obshchei khirurgii,
e-mail: alexeevant@list.ru
Glukhov Alexandr Anatol'evich
Glukhov A.A. MD, professor, a head of the general surgery chair of SBEE HPE “Voronezh State Medical academy named after N.N. Burdenko
Alekseeva N.T. PhD, associate professor of the normal human anatomy chair of SBEE HPE “Voronezh State Medical academy named after N.N. Burdenko
Ostroushko A.P. PhD, assistant of the general surgery chair of SBEE HPE “Voronezh State Medical Academy named after N.N. Burdenko
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