Year 2011 Vol. 19 No 6

GENERAL AND SPECIAL SURGERY

V.G. BOGDAN, YU.M GAIN

PATHOGENESIS OF POSTOPERATIVE HERNIAS: METABOLISM CHANGES OF THE CONNECTIVE TISSUE – CAUSE OR CONSEQUENCE?

Objectives. To establish the nature of metabolic disturbances of the connective tissue in cell cultures of the skin fibroblasts, aponeurosis, and mesenchymal stem cells of the adipose tissue of patients with postoperative ventral hernias.
Methods. The expression of collagen type III and matrix metalloproteinase 1 and 2 types in various cell cultures of patients with postoperative hernias was studied using the method of reverse transcriptase polymerase chain reaction, phenotyping of cell cultures, immuno-cytochemical techniques.
Results. General regularities inherent in primary cultures of cells of mesenchymal origin were determined indicating to the metabolic disturbance of the connective tissue in patients with the postoperative ventral hernias. A single dynamics of the expression of collagen type III decrease and metalloproteinase 1 increase at cells passaging of the fibroplastic differon in vitro was revealed.
Conclusions. The probable cause of metabolic disturbances of the connective tissue in patients with postoperative ventral hernias may be either genetic changes or long-lasting presence of the abdominal wall defect, which determines the increased threshold of the production of mediators, activating the structuring and reducing the remodeling of the connective tissue.

Keywords: postoperative hernia, pathogenesis, collagen, matrix metalloproteinases
p. 29 – 35 of the original issue
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